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2007 | 51 | 3 |

Tytuł artykułu

Prevalence of avian Chlamydophila psittaci in China

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Examinations were carried out in 46 intensive farms in northern China to investigate avian Chlamydophila psittaci. Five hundred and twenty-five avian sera were selected for examining antibodies to C. psittaci by ELISA. One hundred and fifty-five clinical samples from throat swabs and oviduct tissues were tested for the presence of chlamydial antigen using IDEIA™ PCE chlamydia dual amplification immunoassay, and 60 samples were tested by ompA gene-based PCR. C. psittaci antibodies were detected in 387 (77.8%) out of 525 serum samples, with seroprevalences ranging from 50% to 100%. Among the tested samples, 98/150 (65.3%) in broilers, 173/210 (82.3%) in ducks, and 116/165 (70.3%) in laying hens were detected to be positive, respectively. Using PCE-ELISA test kits, in 91 out of 155 clinical samples the presence of antigen was confirmed, while 64 samples were negative. Forty-three PCR's were tested as positive out of 60 samples, while 17 samples were confirmed to be negative. Both higher positive antibodies and the presence of antigens were found in avian flocks associated with typical clinical signs suggestive of chlamydiosis. This study showed a severe prevalence of C. psittaci among different species of domestic birds in China.

Wydawca

-

Rocznik

Tom

51

Numer

3

Opis fizyczny

p.347-350,ref.

Twórcy

autor
  • College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
autor
autor
autor

Bibliografia

  • 1. Andersen A.: Chlamydiosis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens. 4th Edition. Iowa, Kendall/Hunt Publishing, 1998, pp. 34-40.
  • 2. Cao J., Yang Q., Yang L., He. C.: Investigation of avian Chlamydophila psittaci in Beijing and 6 provinces around the capital. Vet Sci China 2006, 36, 931-937.
  • 3. Grimes J E., Wyrick P B.: Chlamydiosis. In: Disease of Poultry. Calnek B.W., Barnes H.J., Beard C.W., Reeid W.M. and Yoder H.W. (ed.).9th edition. Iowa, Iowa State University Press, 1991, pp. 273-283.
  • 4. He C., Shi Y., Duan Q.: Isolation and characterization of a Chlamydia psittaci in broiler. Chinese J Lab Animal Sci 2003, 3, 123-128.
  • 5. Hewinson R.G., Griffiths P.C., Bevan B.J., Kirwan S. E.: Detection of Chlamydia psittaci DNA in avian clinical samples by polymerase chain reaction. Vet Microbiol 1997, 54, 155-166.
  • 6. Kaltenboeck B., Kousoulas K.G., Stroz J.: Two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp. J Clinic Microbiol 1992, 30, 1098-1104.
  • 7. Longbottom D., Coulter L. J.: Animal chlamydioses and zoonotic implications. J Comp Path 2003, 128, 217-244.
  • 8. Prukner-Radovcic E., Horvatek D., Miskovic Z., Mazija H.: Chlamydophilosis in laying hens: a case report. In: COST Action 855, Proceedings of the 4th Workshop: Diagnosis and pathogenesis of animal chlamyidioses. Longbotom D., Rocchi M. (ed.). Meigle Colour Printers Ltd, Edinburgh, 2006, p. 82.
  • 9. Qiu Q. C.: Research progress on avian chlamydiosis. Sci Information Animal Husband Vet Medicine 2002, 8, 12-16.
  • 10. Residbegovic E., Kavazovic A., Gagic A., Kustura A., Goletic T., Vlahovic K., Satrovic E., Dovc A.: The prevalence of chlamydiosis in Bosnia and Herzegovina-preliminary data. In: COST Action 855, Proceedings of the 3rd Workshop: Diagnosis and pathogenesis of animal chlamydioses. Cevennini, R., and Sambr, V. (ed.). Bononia University Press, Bologna, 2005, p. 57.
  • 11. Sayada C., Andersen A.A., Storey C., Milon A.: Usefulness of ompl restriction mapping for avian Chlamydia psittaci isolate differentiation. Res Microbiol 1995, 146, 155-165.
  • 12. Vanrompay D., Butaye P., Sayada C., Ducatelle R., Haesebrouck F.: Characterization of avian Chlamydia psittaci strains using ompl restriction mapping and serovar-specific monoclonal antibodies. Res Microbiol 1997, 148, 327-333.
  • 13. Verminnen K., Van Look M., Hafez H. M., Ducatelle R., Haesebrouck F., Vanrompay D.: Evaluation of a recombinant MOMP-based enzyme-linked immunosrbent assay for detecting Chlamydophila psittaci antibodies in turkey sera. In: COST Action 855, Proceedings of the 4th Workshop: Diagnosis and pathogenesis of animal chlamyidioses. Longbotom D., Rocchi M. (ed.). Meigle Colour Printers Ltd, Edinburgh, 2006, p. 79.

Typ dokumentu

Bibliografia

Identyfikatory

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