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2005 | 27 | 2 |

Tytuł artykułu

Expressed Sequence Tags and their application for plant research

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Expressed Sequence Tags (ESTs) are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from any cDNA library. Analyzing and comparing ESTs can provide information on gene expression, function and evolution. Large-scale EST sequencing has become an attractive alternative to plant genome sequencing. Currently, plant EST coltections comprise over 3.8 miliion sequences from about 200 species. They have proved to be a valuable tool for gene discovery and plant metabolism analysis. Several plant-specific EST databases have been created which provide access to sequence data and bioinformatics-based tools for data mining. Searching EST coltections allows pre-selection of genes for preparing cDNA arrays, targeted to bring maximum information on specialized processes, like stress response, symbiotic nitrogen fixation etc. Also, EST-based molecular markers such as SNP, SSR, and indels are fast developing tools for breeders and re searchers.

Wydawca

-

Rocznik

Tom

27

Numer

2

Opis fizyczny

p.157-161,ref.

Twórcy

autor
  • Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland

Bibliografia

  • Aharoni A., Vorst O. 2001. DNA microarrays for functional plant genomics. Plant Mol. Biol., 48: 99-118
  • Ching A., Rafalski A. 2002. Rapid genetic mapping of ESTs using SNP pyrosequencing and indel analy sis. Cell Mol Biol Lett., 7: 803-810
  • Colebatch G., Kloska S., Trevaskis B., Freund S.,Altmann T., Udvardi M.K. 2002. Novel Aspects of Symbiotic Nitrogen Fixation Uncovered by Transcript Profiling with cDNA Arrays. MPMI, 15: 411-420.
  • Crock J., Wildung M., Croteau R. 1997. Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha xpiperita, L.) that produces the aphid alarm pheromone (E)-beta-farnesene. Proc Natl Acad Sci, 94: 12833-12838.
  • Dong Q., Roy L., Freeling M., Walbot V., Brendel V. 2003. ZmDB, an integrated database for maize genome research. mDB, an iniegrated daiabase for maize ges nome research. Nucleic Acids Res., 31: 244-247.
  • Fedorova M., van de Mortel J., Matsumoto P.A., Cho J. Town Ch.D., VandenBosch K.A., Gantt S., Vance C.P. 2002. Genome-Wide Identification of Nodule-Specific Trans cripts in the Model Legume Medicago truncatula. Plant Physiol., 130: 519-537.
  • Guterman I., Shalit M., Menda N., Piestun D., Dafny-Yelin M., Shalev G., Bar E., Davydov O., Ovadis M., Emanuel M., Wang J., Adam Z., Pichersky E., Lewinsohn E., Zamir D., Vainstein A., Weiss D. 2002. Rose scent: genomics approach to discovering novel floral fragrance-related genes. Plant Cell, 14: 2325-2338.
  • Kantety R.V., La Rota M., Matthews D.E., Sorrells M.E. 2002. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Mol. Biol., 48: 501-510.
  • Mekhedov S., de Ilarduya O.M. Ohlrogge J. 2000. Toward a Funciional Cataiog of the Plant Genome. A Survey of Genes for Lipid Biosynthesis. Plant Physiol., 122: 389-401.
  • Rafalski J.A., Hanafey M., Miao G.H., Ching A., Lee J.M., Dolan M., Tingey S. 1998. New experimental and compuiational approaches to the analysis of gene exi pression. Acta Biochim Pol., 45: 929-934
  • Shoemaker R. et al. 2002. A compilation of soybean ESTs: generation and analysis. Genome, 45: 329-338.
  • Ujino-Ihara T., Yoshimura K., Ugawa Y., Yoshi- maru H., Nagasaka K., Tsumura Y. 2000. Expression analysis of ESTs derived from the inner bark of Crypto- meriajaponica. PlantMol. Biol., 43: 451-45
  • Xu W., Bak S., Decker A., Paquette S.M., Feyereisen R., Galbraith D.W. 2001. Microarray-based analysis of gene expression in very large gene families: the cytochrome P450 gene superfamily of Arabidopsis thaliana. Gene, 272:61-74.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-5faae731-c9d1-4d3d-b1be-97120419de40
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