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2006 | 62 | 08 |

Tytuł artykułu

Izolacja lipidozaleznych szczepow Malassezia od psow i kotow

Warianty tytułu

EN
Isolating lipid-dependent strains Malassezia from dogs and cats

Języki publikacji

PL

Abstrakty

EN
The study investigated 180 clinically healthy dogs and 35 cats with symptoms of otitis externa. 96 strains of Malassezia were isolated, including 13.5% (13 strains) of the lipid-dependent species, and the remainder was classified as M. pachydermatis. Ten lipophilic isolates came from diseased animals, two of which were isolated from dogs. M. globosa (5 strains), M. sympodialis (5 strains), M. furfur (one strain) were isolated within the lipophilic strain pool by using phenotype classification and two isolate species remained unidentified. Genotype identification was performed by PCR-REA (ITS, 26S,Bt) and biochemical identification results for all M. sympodialis and M. globosa strains were confirmed. The M. furfur strain and two isolates of an unrecognizable species were reclassified to M. pachydermatis. Isolating lipid-dependent Malassezia strains from animals having otitis externa is a unique phenomena, and, it seems, that this is the first time in which their isolation and identification from dogs by the use of molecular biology techniques has been described.

Wydawca

-

Rocznik

Tom

62

Numer

08

Opis fizyczny

s.913-916,rys.,tab.,bibliogr.

Twórcy

autor
  • Akademia Rolnicza, ul.Akademicka 12, 20-033 Lublin

Bibliografia

  • 1.Aspiroz C., Ara M., Varea M., Rezusta A., Rubio C.: Isolation of Malassezia globosa and Malassezia sympodialis from patients with pityriasis versicolor in Spain. Mycopathologia 2001, 154, 111-117.
  • 2.Belkum A., Boekhout T., Bosboon R.: Monitoring spreed of Malassezia infections in a neonatal intensive care unit by PCR-mediated genetic typic. J. Clin. Microbiol. 1994, 32, 2528-2538.
  • 3.Boekhout T., Kamp M., Gueho E.: Molecular typing of Malassezia species with PFGE and RAPD. Med. Mycol. 1998, 36, 365-372.
  • 4.Bond R., Anthony R. M., Dodd M., Lloyd D. H.: Isolation of Malassezia sympodialis from feline skin. J. Med. Vet. Mycol. 1996, 34, 145-147.
  • 5.Bond R., Anthony R. M.: Characterization of markedly lipid-dependent Malassezia pachydermatis isolates from healthy dogs. J. Appl. Bacteriol. 1995, 78, 537-542.
  • 6.Bond R., Curtis C. F., Ferguson E. A., Mason J. S., Rest J.: An idiopathic facial dermatitis of Persian cats. Vet. Dermatol. 2000, 11, 35-41.
  • 7.Bond R., Howell S. A., Haywood P. J., Lloyd D. H.: Isolation of Malassezia sympodialis and M. globosa from healthy pet cats. Vet. Rec. 1997, 141, 200- -201.
  • 8.Crespo M. J., Abarca M. J., Cabanes F. J.: Occurence of Malassezia spp. In horses and domestic ruminants. Mycoses 2002, 45, 333-337.
  • 9.Crespo M. J., Abarca M. L., Cabanes F. J.: Isolation of Malassezia furfur from a cat. J. Clin. Microbiol. 1999, 37, 1573-1574.
  • 10.Crespo M. J., Abarca M. L., Cabanes F. J.: Otitis externa associated with Malassezia sympodialis in two cats. J. Clin. Microbiol. 2000, 38, 1263-1266.
  • 11.Crespo M. J., Abarca M. L., Cabanes F. J.: Atypical lipid-dependent Malassezia species isolated from dogs with otitis externa. J. Clin. Microbiol. 2000, 38, 2383-2385.
  • 12.Ekman S.: PCR optimization and troubleshooting with special reference to the amplification of ribosomal DNA in lichenized fungi. Lichenologist 1999, 31, 517-531.
  • 13.Gueho E., Boeghout T., Ashbee H. R., Guillot J., Van Belkum A., Faergemann J.: The role of Malassezia species in the ecology of human skin and as pathogens. Med. Mycology. 1998, 36, 220-229.
  • 14.Guillot J., Bond R.: Malassezia pachydermatis: a review. Med. Mycol. 1999, 37, 295-306.
  • 15.Guillot J., Gueho E., Lesourd M., Midgley G., Chevrier G., Dupont B.: Identification of Malassezia species: A practical approach. J. Mycol. Med. 1996, 6, 133-110.
  • 16.Gupta A. K., Kohli Y., Summerbell R. C.: Molecular differentiation of seven Malassezia species. J. Clin. Microbiol. 2000, 38, 1869-1875.
  • 17.Mayser P., Haze P., Papavassilis C., Mickel M., Gruender K., Gueho E.: Differentiation of Malassezia species: selectivity of Cremophor EL, castor oil and ricinoleic acid for Malassezia furfur. Br. J. Dermatol. 1997, 137, 208- -213.
  • 18.Mirhendi H., Makimura K., Zomorodian K., Yamada T., Sugita T., Yamaguchi H.: A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species. J. Microbiol. Methods 2005, 61, 281-284.
  • 19.Mickelsen P. A., Viano-Paulson M. C., Stevens D. A., Diaz P. S.: Clinical and microbiological features of infection with Malassezia pachydermatis in high- -risk infant. J. Inf. Dis. 1988, 6, 1163-1168.
  • 20.Nowakiewicz A.: Współczesne metody różnicowania diagnostycznego szczepów z rodzaju Malassezia izolowanych z przypadków klinicznych. Rozprawa dokt. Wydz. Medycyny Weterynaryjnej, AR Lublin 2005.
  • 21.Wołoszyn S., Winiarczyk S.: Rola grzybów z rodzaju Pityrosporum w patogenezie schorzeñ skórnych. Medycyna Wet. 1986, 42, 131-135.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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