Immunolocalization of CGRP, NPY and PGP 9.5 in guinea pig skin

• Autorzy: Gendek-Kubiak H , Kmiec B.L.
• Języki publikacji: EN

Abstrakty

EN

By means of immunoperoxidase and immune-alkaline phosphatase methods the immunoreactivities to neuropeptides: neuropeptide Y (NPY), calcitonin gene related peptide (CGRP) and a pan-neuronal marker, the protein gene product 9.5 (PGP 9.5), were evaluated in guinea pig deep dorsal skin specimens. CGRP immunoreactive (CGRP-IR) and NPY-immunoreactive (NPY-IR) nerve fibres were dispersed in the papillary dermis and sometimes inside the hair roots and among the sebaceous gland cells. Such localized nerve fibres have not so far been described. In the subcutaneous layer nerve trunks were found composed of CGRP-IR and NPY-IR nerve fibres. Some of these indicated vestigial or negative immunoreactivity to PGP 9.5.

Słowa kluczowe

EN

calcitonin gene-related peptide; skin; neuropeptide; immunoperoxidase; nerve fibre; protein gene product; guinea pig; immune-alkaline phosphatase; immunoreactivity; pan-neuronal marker; neuropeptide Y

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2004
• Tom: 63
• Numer: 1
• Opis fizyczny: p.115-117,fig.,ref.

Twórcy

autor

Gendek-Kubiak H

• Medical University, Narutowicza 60, 90-136 Lodz, Poland

autor

Kmiec B.L.

Bibliografia

• 1. Ekblad E, Edvinsson L, Wahlestedt C, Uddman R, Hakanson R, Sundler F (1984) Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers. Reg Peptides, 8: 225–235.
• 2. Gray TS, Morley JE (1986) Neuropeptide Y: anatomical distribution and possible function in mammalian nervous system. Life Sci, 38: 389–401.
• 3. Heym C, Liu N, Gleich A, Oberst P, Kummer W (1993) Immunohistochemical evidence for different pathways immunoreactive to substance P and calcitonon generelated peptide (CGRP) in the guinea-pig stellate ganglion. Cell Tiss Res, 272: 563–574.
• 4. Karanth SS, Springall DR, Kuhn DM, Levene MM, Polak JM (1991) An immunocytochemical study of cutaneous innervation and the distribution of neuropeptides in man and commonly employed laboratory animals. Am J Anat, 2191: 369–383.
• 5. Kawasaki H (2002) Regulation of vascular function by perivascular calcitonin gene-related peptide-containing nerves. Jpn J Pharmacol, 88: 39–43.
• 6. Lambert RW, Campton K, Ding W, Ozawa H, Granstein RD (2002) Langerhans cell expression of neuropeptide Y and peptide YY. Neuropeptides, 36: 246–251.
• 7. Majewski M, Heym C (1992) Immunohistochemical localization of calcitonin gene-related peptide cotransmitters in a subpopulation of post-ganglionic neurons in the porcine inferior mesenteric ganglion. Acta Histochem, 92: 138–146.
• 8. Morris JL, Anderson RL, Gibbins IL (2001) Neuropeptide Y immunoreactivity in cutaneous sympathetic and sensory neurons during development of the guinea pig. J Comp Neurol, 437: 321–334.
• 9. Wahlestedt C, Edvinsson L, Ekblad E, Hakanson R (1985) Neuropeptide Y potentiates noradrenaline evoked vasoconstriction: mode of action. J Pharmacol Exp Ther, 234: 735–741.