Growth and survival of rohu, Labeo rohita [Hamilton] spawn fed diets supplemented with fish intestinal microflora

• Autorzy: Ghosh K , Sen S.K. , Ray A.K.
• Języki publikacji: EN

Abstrakty

EN

A 21-day feeding experiment was conducted to assess the effects of diets supplemented with fish intestinal bacteria producing extracellular enzyme on growth and survival of rohu, Labeo rohita (Hamilton) spawn (average weight 0.3 mg). Intestinal bacteria were isolated from healthy rohu fingerlings (average weight 3.51 ±0.31 g). Six isocaloric (4.24 kcalּg-1) and isonitrogenous (approximately 35% crude protein) diets (D1-D6) were prepared containing 32% fish meal, 34% mustard oil cake, 30% rice bran, 2% cod liver oil, and 2% vitamin premix. Experimental diets (D2-D6) were supplemented with the isolated bacterial strain Bacillus circulans Lr 1.1 at five different levels (4 × 105, 4 × 106, 4 × 107, 4 × 108 and 4 × 1010 cells per 100 g of feed, respectively). Rearing of spawn on diet D5 (supplemented with 4 × 108 cells per 100 g) resulted in better growth and survival (97.33 ±1.15%) of rohu spawn compared to those with the control diet (diet D1, without any microflora supplementation) and other experimental diets. Diet D5 resulted in significantly higher growth and specific growth rate (SGR) followed by diets D6 and D4. RNA and DNA contents in the spawn were measured as an index of growth. The correlation coefficient (r value) of 0.831 and regression line having a slope of 0.79 suggested a significant positive correlation (0.01 level) between RNA-DNA ratio and specific growth rate.

PL

Przeprowadzono 21-dniowe doświadczenie dla oceny efektów zastosowania pasz zawierających bakterie jelitowe ryb wytwarzające egzogenne enzymy w żywieniu wylęgu grubowarga, Labeo rohita (Hamilton) (średnia masa 0,3 g). Bakterie jelitowe były wcześniej izolowane ze zdrowego narybku grubowarga (średnia masa 3,51 +0,31 g). Przygotowano sześć izokalorycznych (4,24 kcal*g-1), izoazotowych (w przybliżeniu 35% białka ogólnego) wariantów paszowych (D1-D6) zawierających 32% mączki rybnej, 34% wytłoków gorczycy, 30% otrąb ryżowych, 2% wątroby dorsza oraz 2% premiksu witaminowego. Każdy z wariantów paszowych (D1-D6) wzbogacono wcześniej izolowanym szczepem bakterii Bacillus circulans Lr 1.1 w ilości odpowiednio: 4 x 105, 4 x 106, 4 x 107 , 4 x 108 oraz 4 x 1010 komórek na 100 g paszy. Wylęg żywiony paszą D5 (z dodatkiem 4 x 108 komórek na 100 g) uzyskał lepsze przyrosty masy i miał większą przeżywalność (97,33 +1,15%) w porównaniu do ryb żywionych paszą kontrolną (D1 — bez dodatku bakterii). Zastosowanie paszy D5 spowodowało statystycznie istotnie lepszy dobowy przyrost jednostkowy (SGR). Nieco gorsze okazały się wyniki na paszach D6 i D4. Stosunek RNA i DNA w wylęgu wykorzystano jako wskaźnik wzrostu. Współczynnik korelacji (r) na poziomie 0.831 oraz wskaźnik nachylenia prostej regresji (0.79) wskazują na statystycznie istotną korelacje (p < 0,01) pomiędzy proporcjami RNA-DNA oraz dobowym przyrostem jednostkowym.

Słowa kluczowe

EN

diet; feeding; fish; intestinal microflora; animal physiology; Labeo rohita; aquaculture; growth; rohu; survival

• Wydawca: -
• Czasopismo: Acta Ichthyologica et Piscatoria
• Rocznik: 2002
• Tom: 32
• Numer: 1
• Opis fizyczny: p.83-92,fig.,ref.

Twórcy

autor

Ghosh K

• Visva-Bharati University, Santiniketan - 731 235, West Bengal, India

autor

Sen S.K.

autor

Ray A.K.

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