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1996 | 43 | 1 |

Tytuł artykułu

Expression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression-secretion vector in Saccharomyces cerevisiae

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture conditions. Both cloned genes code for biotechnologically important proteins: human epidermal growth factor and a serine proteinase inhibitor from Cucurbitacea.

Wydawca

-

Rocznik

Tom

43

Numer

1

Opis fizyczny

p.255-264,fig.

Twórcy

  • Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland
autor
autor

Bibliografia

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  • 3. Otlewski, J. (1993) The squash inhibitors of serine proteinases; in Innovations in proteases and their inhibitors (Aviles, F.X., ed.) pp. 369-388, Walter DeGruyter, Berlin. New York.
  • 4. Rempola, B. (1993) Trypsin inhibitor gene cloned and expressed mSacclwromyces cercvisiae. Acta Biochim. Polon. 40. 8-11.
  • 5. Otlewski, J., Zbyrvt, I., Krokoszyriska, I. & Wilusz, T. (1990) Inhibition of serine proteinases by squash inhibitors. Biol. Chew. Hoppe-Scyler 371. 589-594.
  • 6. Birnboim, H.C. & Doly,J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513 1516.
  • 7. Sam brook, J., Fritsch, E.F. & Maniatis, T. (19S9) Molecular cloning. A laboratory manual. Vol.1,2nd edn.. Cold Spring Harbor Laboratory Press, Cold Spring 1 iarbor, N.Y.
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  • 9. Gietz, D.. St. Jean, A., Woods, R.A. & Schiestl, R.H. (1991) Improved method for high efficiency transformation of intact yeast rolls. Nucleic Acids Res. 20, 1425.
  • 10. Savage, C.K., Jr. & Harper, R. (1981) Human epidermal growth factor/urogastrone: rapid purification procedure and partial characte­rization. Anal. Biochcm. Ill, 195-202.
  • 11. Schagger, H. von Jagow, G. (1987) Tricine- sodium dodecyl sulfate-polyacrvlamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 367-379.
  • 12. George-Nascimento, C, Gvenes, A., 1 lalloran, S.M., Merrvweather, J.P., Steimer, K.S., Masiarz, F. R. & Randolph, A. (1988) Characterization of recombinant human epidermal growth factor produced in yeast. Biochemistry 27, 797-802.
  • 13. Otlewski, J., What ley, H., Polanowski. A. & Wilusz, T. (1987) Amino-acid sequences of trypsin inhibitors from watermelon (Citrullus vulgaris) and red bryoni {Bryonia doica) seeds. Biol. Client. Hopj?e-Seyter 368,1505-1507.
  • 14. Pham, T.-C, Konopska-Waliszkiewicz, J. & Leluk, J. (1986) Trypsin inhibitors in the aleurone grains of Cucurbita pepi) var. patissonia (white bush) cotyledons. Biochem. Physiol. Pflanzen 181,565-569.
  • 15. Erlanger, B.F., Kokowsky, N. & Cohen. W. (1961) The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys. 95, 271 278.
  • 16. Filipits, M., Simon, M.M., Rapatz, W., Iiamilton, B. & Ruis, H. (1993) A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation bv fattv acids. Gene 132,49-55.
  • 17. Skoneczny, M. (1993) Regulacja biogenezy peroksysomów i biosyntezy enzymów peroksysomalnych w drożdżach Saccharomyccs cerevisiae. Ph.D. Thesis, IBB, PAN, Warsaw, Poland.
  • 18. Baldari, C., Murray, J. A.H., Ghiara, P., Cesareni, G. &Galeotti,C. (1987) A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1 (3 in Saccharomyces cerevisiae. EMBO J. 6, 229-234.
  • 19. Sleep, D., Belfield, G.P. & Goodey, A.R. (1990) The secretion of human serum albumin from the yeast Saccharonryces cerevisiae using five different leader sequences. BiofTechnology 8, 42-46.
  • 20. Coppella, S.J. & Dhurjati, P. (1989) «-Factor directed expression of the human epidermal growth factor in Saccharomyccs cerevisiae. Biotechnol & Bioeng. 33,976-983.
  • 21. Otlewski, J., Polanowski, A., Leluk. J. & Wilusz, T. (1984) Trypsin inhibitors in summer squash(Citcuriiita f?epo) seeds, isolation, purification and partial characterization of three inhibitors. Acta Biociiim. Poion. 31, 267 278.
  • 22. Miyajima, -V, Bond, M.W., Otsu, K.. Arai, K. Arai, X. {1935) Secretion or mature mouse interleukin-2 by Saccharomyces arevisiae: use of a general secretion vector containing promoter and leader sequences of the matin?, pheromone a-factor. Cent 37, 155-161.
  • 23. Castelli. L.A., Mardon, C.).. Strike. P.M.. Azad. A.A. & Macreadie. l.G. (1994) High-level secretion or correctly processed {}-lactamase from Saccitarontyces cerevisiae using a high-copv-number secretion vector. Can- 142, 113-117.
  • 24. Bitter. G.A., Chen, K.K.. Banks. A.R. £ l.ai, P.-l I (1984; Secretion of foreign proteins from Saccharon/yces cerevisiae directed by a-factor gene fusions. Proc. \at!. Acad, Sci. U.S.A. 81, 5330-5334.
  • 25. Vlasuk, G.P., Bencen, G.l 1., Scarborough. R.M.. Tsai, P.-K., Whang, J.L., Maack, 1., C a mar go, M.j.K, Kirsher, S.W. & Abraham, J.A. (1986) Expression and secretion of biologicallv active human atrial natriuretic peptide in Saccharoniyce* cerevisiae. J. Biol. Chew. 261. 4789-4796.
  • 26. Bourbonnais, Y., Bolin, D. & Shields, L". J988, Secretion of somatostatin by Saccitu-omfiv? cerevisiae. /. Biol. Chew. 263, 15342-15347.
  • 27. Oka.T., Sakamoto, S.. Mivoshi. K.-l., Fuwa. 1. Yoda, K., Yamasaki, M., Tarnura. C. & Mivake. T. (19S5) Synthesis and secretion of human epidermal growth factor bv Escherichia coli. Proc Stttl. Acad. Sci. U.S.A. 82,7212 7216.
  • 28. Brake, A.J.. Merryweather. J.P., Coii, D.G., Heberiein, L'.A.. Masiarz, F.R., Mullenbach, G.l., Urdea, M.S., Valenzuela, P. & Barr, PJ. (1984) a-Factor-directed synthesis and secretion of mature foreign proteins in Sacciiaromyce* cerevisiae. Proc. Nail. Acad. Sci. U.S.A 81. 4642-4646.
  • 29. Clare, J.J., Romanos, M.A., Rayment, F.B., Rowedder, J.E., Smith, M.A., Payne, M.M., Sreekrishna. K. & llenwood, C A. (1991) Production of mouse epidermal growm ractor in yeast: high-level secretion using Pichia panaris strains containing multiple gene copies Gene 105. 205-212.
  • 30. Chen. X.-M., Qian, Y.-W., Chi. C-W., Gsr.. K.-D. /hang, M.-F. & Chen, C.-Q. (1992) Chemical synthesis, molecular cloning, and expression of the gene coding for the Trichosanthes trypsin inhibitor—a squash familv inhibitor. /. Biochem. 112.45-51.
  • 31. Rempoia, B., Wilusz, T., Markiewicz, IV. & Fikus, M. (1995) Synthesis, cloning and expression in Escherichia coli of the gene coding for the trypsin inhibitor from Cucnnnu /v/v. Acta Biochint. Polo/t. 42. 109-114.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-52d4362e-690e-495b-a1ef-3751764e5cbc
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