Pathomorphological and immunohistochemical study of selected markers of tumour cell proliferation in equine sarcoids

• Autorzy: Kasperowicz B. , Rotkiewicz T. , Otrocka-Domagala I.
• Języki publikacji: EN

Abstrakty

EN

The purpose of the study was a pathomorphological and immunohistochemical analysis of tumour cells and connective tissue in equine sarcoids. Investigations were performed using histopathological, ultrastructural, immunohistochemical (PCNA, p53, cytokeratin, vimentin) and histochemical (Ag- NORs) methods. The study was conducted on 50 sarcoids originating from 36 horses and classified as occult, verrucous, fibroblastic and a mixed type of sarcoid based on their clinical appearance. Most of the tumours were located on the girth (30%), neck (24%), head (12%), and legs (12%). The average age of the horses at the first clinical examination was 5.7 years. The sarcoids occurred on the skin of mares (61%), geldings (31%) and stallions (8%), the predominant was Wielkopolska breed (41%) and mixed breeds with Wielkopolska breed (41%). The predominant colour was bay (80%). The data showed that the presence of characteristic, microscopic features was variable but it was not consistent enough to allow differentiation of the clinical types based on histopathology. PCNA expression was not characteristic for the clinical type of sarcoid but it appeared to be a useful tool for the determination of the biological activity of the tumour and the probability of its recurrence. No relationship was found between AgNORs and cell proliferation. The study demonstrated the presence of p53 positive cells in the epidermal and fibroblastic portions. Numerous p53-positive cells were observed in the sarcoids and tended to recurrence. The staining for cytokeratin and vimentin makes the diagnosis of tumour easier. The immunohistochemical studies of PCNA, and p53 are of great significance to the prognosis.

Słowa kluczowe

EN

horse; pathomorphology; proliferation; tumour cell; veterinary medicine; cytokeratin; immunohistochemistry; vimentin; tumour; equine sarcoid

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2006
• Tom: 09
• Numer: 2
• Opis fizyczny: p.109-119,fig.,ref.

Twórcy

autor

Kasperowicz B.

• University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Rotkiewicz T.

autor

Otrocka-Domagala I.

Bibliografia

• Angelos JA, Marti E, Lazary S, Carmichael LE (1991) Char­acterization of BPV-like DNA in equine sarcoids. Arch Virol 119: 95-109.
• Baker JR, Leyland A (1975) Histological survey of tumours of the horse, with particular reference to those of the skin. Vet Rec 96: 419-422.
• Bastianello SS (1983) A survey on neoplasia in domestic species over a 40-year period from 1935 to 1974 in the Republic of South Africa. IV. Tumours occurring in Equidae. Oderstepoort J Vet Res 50: 91-96.
• Brostrom H (1995) Equine sarcoids. A clinical and epi­demiological study in relation to equine leucocyte anti­gens (ELA). Acta Vet Scand 36: 223-236.
• Brostrom H, Fahlbrink E, Dubath ML, Lazary S (1988) As­sociation between equine leucocyte antigens (ELA) and equine sarcoid tumors in the population of Swedish halfbreds and some of their families. Vet Immunol Im- munopathol 19: 215-223.
• Bucher K, Szalai G, Marti E, Griot-Wenk ME, Lazary S (1996) Tumour suppressor gene p53 in the horse: identification, cloning, sequencing and a possible role in the pathogenesis of equine sarcoid. Res Vet Sci 61: 114-119.
• Carr EA, Theon AP, Madewell BR, Griffey SM, Hitchcock ME (2001) Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States. Am J Vet Res 62: 741-744.
• Cheevers WP, Fatemi-Nainie S, Anderson LW (1986) Spon­taneous expression of an endogenous retrovirus by the equine sarcoid-derived Mc-1 cell line. Am J Vet Res 47: 50-52.
• Hall PA, Levison DA, Woods AL, Yu CC-W, Kellock BD, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R, Waseem NH, Lane DP (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162: 285-294.
• Laursen BA (1987) Cryosurgery as treatment of equine sar­coids. Dansk Vet Tidskr 70: 97- 104.
• Lazary S, Marti E, Szalai G, Gaillard C, Gerber H (1994) Studies on the frequency and associations of equine leu­cocyte antigens in sarcoid and summer dermatitis. Anim Genet 25 Suppl 1: 75-80.
• Lory S, von Tscharner C, Marti E, Bestetti G, Grimm S, Waldvogel A (1993) In situ hybridisation of equine sar­coids with bovine papilloma virus. Vet Rec 132: 133-134.
• Madewell BR, Munn RJ (1989) Tubuloreticular inclusions in equine connective tissue neoplasms. J Comp Pathol 100: 449-452.
• Martens A, de Moor, A, Demeulemeester J, Ducatelle R (2000) Histopathological characteristics of five clinical types of equine sarcoid. Res Vet Sci 69, 295-300.
• Marti E, Lazary S, Antczak DF, Gerber H (1993) Report of the first international workshop on equine sarcoid. Equine Vet J 25: 397-407.
• Meredith D, Elser AH, Wolf B, Soma LR, Donawick WJ, Lazary S (1986) Equine leukocyte antigens: relationship with sarcoid tumors and laminitis in two pure breeds. Immunogenetics 23: 221-225.
• Miller RI, Campbell RS (1982) A survey of granulomatous and neoplastic diseases of equine skin in North Queens­land. Aust Vet J 59: 33-37.
• Mohammed HO, Rebhun WC, Antczak DF (1992) Factors associated with the risk of developing sarcoid tumours in horses. EquineVet J 24: 165-168.
• Moll R, Franke WW, Schiller D (1982) The catalog of hu­man cytokeratins: patterns of expression in normal epi- thelia, tumors and cultured cells. Cell 31:11-16.
• Momand M, Zambetti GP, Olson DC, George D, Levine KJ (1992) The mdm-2 oncogene product forms a complex with p53 protein and inhibits p53 mediated transactiva­tion. Cell 69: 1237-1245.
• Nasir L, Reid SW (1999) Bovine papillomaviral gene ex­pression in equine sarcoid tumours. Virus Res 62: 171-175.
• Ostrowski K (1995) Histologia (in Polish). PZWL, War­szawa
• Ploton D (1994) Structure and molecular organization of the nucleous. Zentralbl Pathol 140: 3-6.
• Ragland WL, Keown GH, Spencer GR (1970) Equine sar­coid. Equine Vet J 2:2-11.
• Ragland WL, Keown GH, Gorham JR (1966) An epizootic of equine sarcoid. Nature 25: 1399.
• Reid SWJ, Mohammed HO (1997) Longitudinal and cross-sectional studies to evaluate the risk of sarcoid asso­ciated with castration. Can J Vet Res 61: 89-93.
• Teifke JP, Hardt M, Weiss E (1994) Detection of bovine papillomavirus DNA in formalin-fixed and parafin-em- bedded equine sarcoid by polymerase chain reaction and non-radioactive in situ hybridization. Eur J Vet Pat 1: 5-10.
• Torrontegui BO, Reid SJ (1994) Clinical and pathological epidemiology of the equine sarcoid in a referral popula­tion. Equine Vet Educ 6: 85-88.
• Trenfield K, Spradbrow PB, Vanselow B (1985) Sequences of papillomavirus DNA in equine sarcoids. Equine Vet J 17: 449-452.
• Werness BA, Levine AJ (1990) Association of human papil­lomavirus types 16 and 18 E6 proteins with p53. Science 248: 76-79.
• Williams IF, Heaton A, McCullagh KG (1982) Connective tissue composition of equine sarcoid. Equine Vet J 14: 305-310.
• Wiśniewski E, Janiszewski J (1994) Nowotwory skóry koni. Med Weter 50: 212-215.