Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits

• Autorzy: Huang Y-Z , Zhang E-P , Chen H , Wang J , Li Z-J , Huai Y-T , Ma L , Lan X-Y , Ren G , Lei C-Z , Fang X-T , Wang J-Q
• Języki publikacji: EN

Abstrakty

EN

The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and Polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P <0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.

Słowa kluczowe

PL

cattle; growth trait; deletion mutation; NPM1 gene; bovine gene; coding region; candidate gene; phosphoprotein; nucleolus; cellular process

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 2
• Opis fizyczny: p.199-202,fig.,ref.

Twórcy

autor

Huang Y-Z

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Zhang E-P

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Chen H

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Wang J

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Li Z-J

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Huai Y-T

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Ma L

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Lan X-Y

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Ren G

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Lei C-Z

• College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, China

autor

Fang X-T

• Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, China

autor

Wang J-Q

• Research Center of Cattle Engineering Technology in Henan; Zhengzhou Henan, China

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