Organogenesis initiation and plant regeneration from hypocotyls and cotyledones of androgenic embryos of Brassica napus L.

• Autorzy: Bagniewska-Zadworna A. , Cegielska-Taras T. , Zenkteler M.
• Języki publikacji: EN

Abstrakty

EN

Regeneration of plants from hypocotyls and cotyledons of androgenic embryos of winter oilseed rape (Brassica napus L. homozygous line DH-O 120) were investigated. Both types of explants were cultured on Murashige and Skoog (MS) and Gamborg (B5) media with or without a low concentration of growth regulators added. Buds showed the highest developmental ability on B5 medium in the presence of 0.5 mg 1-1GA3**. All shoots and secondary embryos derived from cotyledons and hypocotyls were of cortical origin. Starch grains gradually disappeared as shoot initiation proceeded, indicating their role in the process of organogenesis.

Słowa kluczowe

EN

oilseed rape; plant regeneration; Brassica napus; cotyledon; seedling; organogenesis; haploid; hypocotyl; androgenic embryo

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2001
• Tom: 43
• Opis fizyczny: p.51-57,fig.,ref.

Twórcy

autor

Bagniewska-Zadworna A.

• Adam Mickiewicz University, Al.Niepodleglosci 14, 61-713 Poznan, Poland

autor

Cegielska-Taras T.

autor

Zenkteler M.

Bibliografia

• Bogunia H, and Przywara L. 2000. Effect of carbohydrates on callus induction and regeneration ability in Brassica napus L. Acta Biologica Cracoviensia Series Botanica 42/1: 79-86.
• Broda B. 1971. Metody histochemii roślinnej. PWZL, Warszawa.
• Cegielska-Taras T, and Szała L. 1997. Plants regeneration from microspore-derived embryos of Brassica napus L. Oilseed Crops XVIII: 21-30.
• Cegielska-Taras T, and Szała L. 1998. Direct production of doubled haploid plants from microspore-derived embryos of winter oilseed rape (Brassica napus L.). Oilseed Crops XIX: 353-357.
• Cegielska-Taras T, Szała L, Nałęczyńska A, Kołodziej K, and Ogrodowczyk M. 1999. Selection for high erucic acid content in winter oilseed rape (Brassica napus L.) on microspore-derived embryos. Journal Applied Genetics 40: 305-315.
• Chuong PV, and Beversdorf WD. 1985. High frequency embryogenesis through isolated microspore culture in Brassica napus L. and B. carinata Braun. Plant Science 39: 219-226.
• Gamborg O, Miller R, and Oijima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 150: 151-158.
• Gland A, Lichter R, and Schweiger HG. 1988. Genetic and exogenous factors affecting embryogenesis in isolated microspore cultures of Brassica napus L. Journal Plant Physiology 132: 613-617.
• Iwanowska A, Chomicz B, Tykarska T, and Kuraś M. 1997. Microspore-derived embryos of Brassica napus L. cv. Topas and their conversion. Oilseed Crops XVIII: 1-6.
• Koh WL, and Loh CS. 2000. Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid cycling Brasstca napus. Plant Cell Reports 19: 1177-1183.
• Kott LS, and Beversdorf WD. 1990. Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling partial desiccation and age selection. Plant Cell, Tissue and Organ Culture 23: 187-192.
• Murashige J, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473-497.
• Pechan PN, and Keller WA. 1988. Identification of potentially embryogenic microspores in Brassica napus. Physiologia Plantarum 74: 377-384.
• Rogozińska JH, and Drozdowska L. 1980. Organogenesis and plant formation from cotyledon and callus culture of rape. Acta Societatis Botanicorum Poloniae 49: 5-20.
• Sharma K, Bhojvani SS, and Thorpe TA. 1991. The role of cotyledonary tissue in the differentiation of shoots and roots from cotyledon explants of Brassica juncea L. Plant Cell, Tissue and Organ Culture 24: 55-59.
• Takahata Y, Braun DCW, and Keller WA. 1991. Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. Euphytica 58: 51-55.
• Thomas E, and Wenzel G. 1975. Embryogenesis from microspore culture of Brassica napus. Zeitchrift für Pflanzenzüchtung 74: 77-81.
• Tykarska T. 1979. Rape embryogenesis. II. Development of embryo proper. Acta Societatis Botanicorum Poloniae 48: 391- 421.
• Tykarska T. 1982. Rape embryogenesis. IV. Appearance and disappearance of starch during embryo development. Acfa Societatis Botanicorum Poloniae 51: 381—387.
• Tykarska T. 1987. Rape embryogenesis. V. Accumulation of lipid bodies. Acta Societatis Botanicorum Poloniae 56: 573-584.
• Wojciechowski A. 1998. Regeneration abilities of chosen genotypes of Brassica in in vitro cultures. Scientific Papers of the Agricultural University of Poznań. 289.