PL
Pacjenci hospitalizowani na oddziałach intensywnej terapii (OIT) są szczególnie narażeni na zakażenia wywołane przez bakterie oporne na wiele antybiotyków. Celem pracy była ocena częstości występowania i lekowrażliwości szczepów pałeczek Gram-ujemnych z rodziny Enterobacteriaceae izolowanych z materiałów klinicznych od tej grupy chorych, ze szczególnym uwzględnieniem profilu oddziału. Stwierdzono różnice w obrazie lekowrażliwości zależnie od rodzaju materialu klinicznego i profilu oddziału OIT.
EN
The aim of the study was estimation of frequency and susceptibility to antimicrobial agents of Gram-negative rods isolated from clinical specimens obtained from patients requiring intensive care, with emphasis on profile of the unit. The analysis comprised strains of Gram-negative rods isolated from patients of two intensive care units (ICUs) of a tertiary care hospital (1200 beds). Identification of cultured isolates was done using automated VITEK and API systems (bioMerieux, France). Susceptibility to antimicrobial agents was tested by a disk-diffusion method according to the NCCLS recommendations. In total the analysis comprised 722 strains of Gram-negative rods. In blood cultures predominated strains of Enterobacter spp. (42,5%) and Klebsiella pneumoniae (37,5%). In cultures of clinical specimens other than blood 41,6% comprised strains of Klebsiella pneumoniae, 14,8% Escherichia coli and 14,4% Proteus mirabilis. Frequency of multi-drug resistant strains of bacteria of the family Enterobacteriaceae was much higher among blood isolates in comparison to strains cultured from other clinical specimens. There was a relatively high percentage of strains of Enterobacteriaceae susceptible to piperacillin and tazobactam (69,0%) and ceftazidime(54,6%). Conclusions: 1. All strains were susceptible to carbapenems. 2. There was a relatively high percentage of strains of Gram- negative rods susceptible to piperacillin/tazobactam and ceftazidime. 3. Bacteria isolated from blood cultures were characterised by a much higher percentage of resistant strains in comparison to other specimens. 4. Longer stay in ICU promoted selection of strains resistant to antimicrobials.