Control of salivary phospholipid content and composition

• Autorzy: Slomiany B L , Sengupta S. , Piotrowski E. , Lopez R.A. , Slomiany A.
• Języki publikacji: EN

Abstrakty

EN

The mediation of phospholipid secretion in rat sublingual salivary gland cells maintained in the presence of [³H]choline was investigated. The secretion of [³ H]choline-containing phospholipids was enhanced by β-adrenergic agonist, isoproterenol, to a greater extent than the cholinergic agoinst carbachol. A 2.9-fold increase in phospholipid secretion occurred with isoproterenol, while carbachol evoked only about 1.3-fold increase. In contrast to carbachol, the enhanced phospholipid secretion due to isoproterenol was accompanied by an increase in cAMP concentration. The secretion of phospholipids was also stimulated by dibutyryl-cAMP and the protein kinase C activator, phorbol myristate acetate, but not by 4a-phorbol 12, 13-didecanoate which does not activate protein kinase C. Furthermore, the effects of dibutyryl-cAMP and phorbol myristate acetate were additive. The phospholipids secreted in response to isoproterenol exhibited a 52% decrease in lysophosphatidylcholine, while those secreted in response to carbachol showed a 23% lower content of phosphatidylcholine, and were enriched in lysophosphatidylcholine (2.8-fold) and sphingomyelin (1.4-fold). The results suggest that salivary phospholipid secretion remains mainly under β-adrenergic control, while the phospholipid makeup is under cholinergic regulation.

Słowa kluczowe

EN

cell isolation; cholinergic effect; salivary phospholipid; lipid; cell incubation; saliva; mediation; rat; secretion; adrenergic effect

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1992
• Tom: 43
• Numer: 3
• Opis fizyczny: p.223-235,fig.

Twórcy

autor

Slomiany B L

• University of Medicine Dentistry of New Jersey, Newark, NJ 07103-2400, USA

autor

Sengupta S.

autor

Piotrowski E.

autor

Lopez R.A.

autor

Slomiany A.

Bibliografia

• 1. Slomiany BL, Murty VLN,Slomiany A. Salivary lipids in health and disease. Prog Lipid Res 1985; 24: 311-324.
• 2. Slomiany BL, Aono M, Murty VLN, Slomiany A, Levine MJ, Tabak LA. Lipid composition of the submandibula saliva from normal and cystic fibrosis individuals. J Dent Res 1982; 61: 1163-1166.
• 3. Slomiany BL, Kosmala M, Nadziejko C, et al. Lipid composition and viscosity behavior of partoid saliva in Sjogren syndrome in man. Arch Oral Biol 1986; 31: 699-702.
• 4. Slomiany BL, Murty VLN, Mandel ID, Zalesna G,Slomiany A. Physicochemical characteristics of mucus glycoproteins and lipids of the human mucosal mucus coat in relation to caries susceptibility. Arch Oral Biol 1989; 34: 229-237.
• 5. Slomiany BL,Slomiany A, Mandel ID. Lipids of saliva and salivary concretions. In: Tenovuo J, ed. Clinical Chemistry of Human Saliva. CRC Press. 1989; vol II: 121-145.
• 6. Slomiany BL, Murty VLN, Piotrowski J,Slomiany A. Role of associated and covalently bound lipids in salivary mucin hydrophobicity: effect of proteolysis and disulfide bridge reduction. Biochem Biophys Res Commun 1988; 151: 1046-1053.
• 7. Dirksen TR. Salivary lipids in salivary and dental caries. In: Kleinberg I, ed. Microbiology Abstracts. New York: Information Retrieval 1979; 113-122.
• 8. Garrett JR. Adventures with autonomic nerves. Perspectives in salivary glandular innervations. Proc R Micro Soc 1982; 17: 242-253.
• 9. Baum BJ. Neurotransmitter control of secretion. J Dent Res 197; 66: 628-632.
• 10. Baum BJ. Regulation of salivary system. In: Sreebny LM, ed. The Salivary System: CRS Press. 1987; 123-134.
• 11. Martinez JR. Ion transport and water movement. J Dent Res 1987; 66: 638-647.
• 12. Quissell DO, Barzen KA. Secretory response of dispersed rat submandibular cells. II mucin secretion. Am J Physiol 1980; 238: C99-Cl06.
• 13. McPherson MA, Dormer RL, Bradbury NA, Dodge JA, Goodchild MC. Defective β-adrenergic secretory responses in submandibular acinar cells from cystic fibrosis patients. Lancet 1986; 11: 1007-1008.
• 14. Bradbury NA, McPherson MA. Actions of prostaglandins E₂ and F₂a on release of ¹⁴C-labeled mucins from rat submandibular acini in vitro. Arch Oral Biol 1987; 32: 719-722.
• 15. Doughney C, Dormer RL, McPherson MA. Adrenergic regulation of formation of inositol phosphates in rat submandibular acini. Biochem J 1987; 241: 705-709.
• 16. Quissell DO, Barzen KA, Deisher LM. Evidence against a direct role for protein kinase C in rat submandibular salivary mucin secretion. Arch Oral Biol 1989; 34: 695-699.
• 17. Fleming N, Bilan PT, Sliwinski-Lis E, Carvalho V. Muscarinic α₁ -adrenergic and peptidergic agonists stimulate phosphoinositide hydrolysis and regulate mucin secretion in rat submandibular gland cells. Pflügers Arch 1987; 409: 416-421.
• 18. Brown LAS, Longmore WJ. Adrenergic and cholinergic regulation of lung surfactant secretion in the isolated perfused rat lung and in the alveolar type II cell in culture. J Biol Chem 1981; 256: 66-72.
• 19. Sano K,Voelker DR, Mason RJ. Involvement of protein kinase C in pulmonary surfactant secretion from alveolar type II cells. J Biol Chem 1985; 260: 12725-12729.
• 20. Pritchard ET. Submandibular salivary gland lipid metabolism in the rat: incorporation of ¹⁴C-labeled fatty acid into lipids of slice and homogenate system. Arch Oral Biol 1970; 15: 879-881.
• 21. Fanestil DD, Barrows CH. Aging in the rotifer. J Gerontol 1965; 20: 462-469.
• 22. Sengupta S, Fine J, Wu-Wang CY, et al. The relationship of prostaglandins to cAMP, IgG, IgM and α-2-macroglobulin in gingival crevicular fluid in chronic adult periodontitis. Arch Oral Biol 1990; 35: 593)-596.
• 23. Slomiany BL, Murty VLN, Zdebska E,Slomiany A, Gwozdzinski K, Mandel ID. Tooth-surface pellice lipids and their role in the protection of dental enamel against lactic acid diffusion in man. Arch Oral Biol 1986; 31: 187-191.
• 24. Bilski J, Murty VLN, Aono M, Moriga M,Slomiany A,Slomiany BL. Enhancement of the lipid content and physical properties of gastric mucus by geranylgeranylacetone. Biochem Pharmacol 1987; 26: 4059-4065.
• 25. Pryhuber GS, Reilly MA, Clark JC, Hull WM, Fink I, Whitsett JA. Phorbol ester inhibits surfacant protein SP-A and SP-B expression. J Biol Chem 1986; 265: 20822-20828.
• 26. Slomiany BL, Murty VLN, Liau YH,Slomiany A. Animal glycoglycerolipids. Prog Lipid Res 1987; 26: 29-51.
• 27. Murty VLN,Slomiany BL, Zdebska E,Slomiany A, Mandel ID, Levy BM. Lipid composition of marmoset saliva. Comp Biochem Physiol 1984; 79A: 41-44.
• 28. Slomiany BL, Murty VLN, Mandel ID, Sengupta S,Slomiany A. Effect of lipids on the lactic acid retardation capacity of tooth enamel and cementum pellicles formed in vitro from saliva of caries-resistant and caries-susceptibility human adults. Arch Oral Biol 1990; 35: 175-180.
• 29. Cohen P. Protein phosphorylation and hormone action. Proc R Soc Lond fBiol) 1988; 234: 115-144.
• 30. Exton JH. Signaling through phosphatidylcholine breakdown. J Biol Chem 1990; 265: 1-4.
• 31. Dennis EA, Rhee SG, Billah MM, Hannum YA. Role of phospholipases in generating lipid second messengers in signal transduction. FASEB J 1991; 5: 2068-2077.
• 32. Ho AK, Klein DC. Activation of α₁-adrenoceptors, protein kinase C, or treatment with intracellular free Ca²⁺ elevating agents increases pineal phospholipase A₂ activity. J Biol Chem 1987; 262: 11764-11770.
• 33. Schachtele CF, Harlander SK, Bracke JW, Ostrum LC, Maltais JB, Billings RJ. Streptococcus mutans dextransucrase: stimulation by phospholipids from human sera and oral fluids. Infect Immun 1976; 22: 714-720.