Pollination with heavily irradiated pollen in Nicotiana: induced parthenogenesis and embryological study

• Autorzy: Musial K. , Przywara L.
• Języki publikacji: EN

Abstrakty

EN

Nicotiana crosses were pollinated in situ and in vitro with heavily irradiated pollen (500, 700, 1000 Gy) to induce parthenogenesis and to study the development of embryo and endosperm. Haploids were obtained after in situ pollination only; however, parthenogenetic proembryos occurred also after in vitro pollination. It was demonstrated that ovule culture following pollination offers a better chance to produce haploids than undisturbed pollination does. Pollination with irradiated pollen (PwIP) strongly decreased the number of endosperm cells and the size of embryo sacs, and it affected the development of embryos; no significant differences between applied irradiation doses were found. Ovules with endosperm only, embryo only, and with both embryo and endosperm were observed. The most frequent were the ovules with endosperm only, the rarest with embryo only. A small amount of storage products occurred in the endosperm cells. The diploid chromosome number counted in the endosperm produced after PwIP points to their origin without fertilization. An interesting phenomenon observed after PwIP was vigorous growth of endothelium.

Słowa kluczowe

EN

in situ; embryological analysis; haploid plant; pollination; haploid; in vitro; induced parthenogenesis; Nicotiana; fertilization; pollen irradiation; endosperm; haploid induction; parthenogenesis; endothelium; embryo

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1999
• Tom: 41
• Opis fizyczny: p.127-137,fig.

Twórcy

autor

Musial K.

• Jagiellonian University, ul.Grodzka 52, 31-044 Cracow, Poland

autor

Przywara L.

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