Participation of endoplasmic reticulum in the unequal distribution of plastids during generative cell formation in Gagea lutea [L.] Ker.-Gaw. [Liliaceae]

• Autorzy: Bohdanowicz J. , Lewandowska B.
• Języki publikacji: EN

Abstrakty

EN

The distribution of plastids at the time of microspore and pollen grain development in Gagea lutea (L.) Ker.-Gaw. was analyzed using electron microscopy. It was shown that plastids are not transmitted to the forming generative cell of this species during microspore division. At the vacuolate microspore stage, preceding division, the microspore nucleus takes an acentric position and the plastids gather at the opposite side of the cell. In the highly polarized microspore at prophase of mitosis, all plastids are aggregated at one side of the nucleus, whereas mitochondria are dispersed throughout the cytoplasm. Numerous profiles of endoplasmic reticulum (ER) are present between the clustered plastids. Some of the ER profiles are attached by their ends to the outer membrane of plastid envelopes and join the distant plastids. The outer membrane of the microspore plastids may form long and thin evaginations contacting with other plastids. Microtubules are visible in plastid aggregations occasionally. In dividing microspores, long ER cisterns surround the area of the mitotic spindle and separate it from the region containing plastids. There are no plastids in the young generative cell: all plastids remain clustered in the region of the microspore that now forms the vegetative cell of the bicellular pollen grain. Later the connections between plastids and ER cisterns gradually disappear and plastids disperse in the cytoplasm of the whole vegetative cell. The results of our study are not sufficient to define the mechanism causing selective aggregation of plastids at the vegetative pole of the Gagea microspore, nor to say whether the microtubular cytoskeleton plays a role. However, the participation of ER in these processes, at least in holding the special arrangement of microspore plastids, seems certain.

Słowa kluczowe

EN

pollen grain; plant cell; cytoplasm; vegetative cell; endoplasmic reticulum; Gagea lutea; mitochondrion; membrane; Liliaceae; microtubular cytoskeleton; ultrastructure; microspore mitosis; plastid distribution

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1999
• Tom: 41
• Opis fizyczny: p.177-183,fig.

Twórcy

autor

Bohdanowicz J.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

autor

Lewandowska B.

Bibliografia

• Brown RC, and Lemmon BE. 1991a. Pollen development in orchids. 3. A novel generative pole microtubule system predicts unequal pollen mitosis. Journal of Cell Science 99: 273-281.
• Brown RC, and Lemmon BE. 1991b. Pollen development in orchids. 5. A generative cell domain involved in spatial control of the hemispherical cell plate. Journal of Cell Science 100: 559-565.
• Hagemann R. 1979. Genetics and molecular biology of plastids of higher plants. Stadler Symposia 11: 91-116.
• Hagemann R. 1981. Unequal plastid distribution during the development of male gametophyte of angiosperms. Acta Societatis Botanicorum Poloniae 50: 321-327.
• Hagemann R, and Schröder M-B. 1989. The cytological basis of plastid inheritance in Angiosperms. Protoplasma 152: 57-64.
• Hepler P, Palevitz B, Lancelle S, Mc Cauley M, and Lichtscheidl I. 1990. Cortical endoplasmic reticulum in plants. Journal of Cell Science 96: 355-373.
• Kirk JTO, and Tilney-Bassett RAE. 1978. The plastids. Their chemistry, structure, growth and inheritance. Elsevier, North Holland, Amsterdam.
• Köhler RH, Cao J, Zipfel WR, Webb WW, and Hanson MR. 1997. Exchange of protein molecules through connections between higher plant plastids. Science 276: 2039-2042.
• Krupko S. 1926. Les plastides et le chondriome pendant la gonogénèse dans le Gagea lutea. Acta Societatis Botanicorum Poloniae 4: 77-86.
• Pacini E, and Cresti M. 1976. Close association between plastids and endoplasmic reticulum cisterns during pollen grain development in Lycopersicon peruvianum. Journal of Ultrastructure Research 57: 260-265.
• Pacini E, and Juniper B. 1984. The ultrastructure of pollen grain development in Lycopersicum peruvianum. Caryologia 17: 21-50.
• Schröder M-B. 1985. Ultrastructural studies on plastids of generative and vegetative cells in Liliaceae. 3. Plastids distribution during the pollen development in Gasteria verrucosa Mill.) Duval. Protoplasma 102: 123-129.
• Schröder M-B. 1986. Ultrastructural studies on plastids of generative and vegetative cells in Liliaceae. 5. The behaviour of plastids during pollen development in Chlorophytum comosum (Thunb.) Jacques. Theoretical and Applied Genetics 72: 840-844.
• Schröder M-B, Van Lammeren AAM, and Kieft H. 1988. The intracellular arrangement of cytoskeletal components and organelles in pollen grains of Gasteria verrucosa (Mill.) H. Duval. Annales de l'Universite et de l'A.R.E.R.S., Reims, 23: 80.
• Sears BB. 1980. Elimination of plastids during spermatogenesis and fertilization in the plant kingdom. Plasmid 4: 233-255.
• Spurr AR. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26: 31-43.
• Tanaka 1.1991. Microtubule-determined plastid distribution during microsporogenesis in Lilium longiflorum. Journal of Cell Science 99: 21-31.
• Van Lammeren AAM, Keijzer CJ, Willemse MTM, and Kieft H. 1985. Structure and functions of the microtubular cytoskeleton during pollen development in Gasteria verrucosa (Mill.) H. Duval. Planta 165: 1-11.
• Van Went L. 1984. Unequal distribution of plastid during generative cell formation in Impatiens. Theoretical and Applied Genetics 68: 305-309.
• Zhang H-Q, Bohdanowicz J, Pierson ES, Li Y-Q, Tiezzi A, and Cresti M. 1995. Microtubular organization during asymmetrical division of the generative cell in Gagea lutea. Journal of Plant Research 108: 269-276.