Cholinergic innervation of cystic porcine ovaries

• Autorzy: Kozlowska A , Wojtkiewicz J. , Majewski M. , Jana B.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to examine the changes in the density of VAChT (marker of acetylcholine present)-, NPY-, VIP-, SOM-, SP- and nNOS-immunoreactive (IR) nerve terminals and co-localization of VAChT with the above-mentioned neurotransmitters after the occurrence of dexamethasone (DXM)-induced ovarian cysts in gilts. DXM administration led to an increase in the density of VAChT/SP-, VAChT/nNOS- and NPY-IR nerve terminals around the cystic walls. In DXM-treated animals an elevated number of VAChT- and SP-IR nerve endings was found close to the tertiary follicles. Moreover, in the gilts receiving DXM the density of NPY-IR nerve endings (that simultaneously co-localized VAChT) was high near the interstitial gland. An increase in the number of VAChT/SP- and VIP-IR nerve fibers around the medullar arteries (A) was observed in cystic ovaries, while the number of VAChT-IR nerve endings near the cortical A was lowered after DXM application. Furthermore, nerve fibers containing VAChT were absent around veins in the whole ovary of DXM-treated animals. After DXM injections, an increase in the number of VAChT/SP- and VAChT/nNOS-IR nerve endings in the cortical, as well as VIP- and nNOS-IR (co-existing with VAChT), nerve terminals in the medullar part of the autonomic ground plexus (GP) was present. However, the administration of DXM led to a drop in the density of SOM-positive nerve endings (also VAChT-IR) in the medullar subdivision of the GP. The present study shows that in the porcine ovaries with DXM induced cysts the pattern of cholinergic innervation, as well as the co-localization of VAChT and NPY, VIP, SOM, SP or nNOS, were changed. Data obtained also suggest that acetylcholine and the above-mentioned neurotransmitters effecting the functioning (steroidogenic activity, blood flow) of the polycystic ovaries may have a significant influence on the course of this pathological status.

Słowa kluczowe

EN

acetylcholine; pig; cystic ovary; cholinergic innervation; cystic ovarian disease; dexamethasone

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 04B
• Opis fizyczny: p.565-570,fig.,ref.

Twórcy

autor

Kozlowska A

• Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

autor

Wojtkiewicz J.

autor

Majewski M.

autor

Jana B.

Bibliografia

• 1.Andreani C. L., Lazzarin N., Pierro E., Lanzone A., Mancuso S.: Somatostatin action on rat ovarian steroidogenesis. Hum. Reprod. 1995, 10, 1968-1973.
• 2.Arancibia S., Estupina C., Tapia-Arancibia L.: Rapid reduction in somatostatin mRNA expression by hypothalamic neurons induced by dexamethasone. Neuroendocrinology 2000, 11, 3163-3167.
• 3.Babalola G. O., Shapiro B. H.: Sex steroid changes in porcine cystic ovarian disease. Steroids 1990, 55, 319-324.
• 4.Balen A. H., Jacobs H. S.: A prospective study comparing unilateral and bilateral laparoscopy ovarian diathermy in women with the polycystic ovary syndrome. Fertil. Sterol. 1994, 62, 921-924.
• 5.Baranowska B., Chmielowska M., Radzikowska M., Borowiec M., Roguski K., Wasilewska-Dziubinska E.: Effects of neuropeptide Y (NPY), galanin and vasoactive intestinal peptide (VIP) on pituitary hormone release and ovarian steroidogenesis. Neuroendocrinol Lett. 1999, 20, 385-389.
• 6.Barboni B., Martelli A., Berardinelli P., Russo V., Turriani M., Bernabò N., Lucidia P., Mattioli M.: Ovarian follicle vascularization in fasted pig. Theriogenology 2004, 62, 943-997.
• 7.Bódis J., Tinneberg H. R., Papenfuss F., Török A., Cledon P., Hanf V., Schwarz H.: Cholinergic stimulation of progesterone and estradiol secretion by human granulosa cells cultured in serum-free medium. Gynecol. Endocrinol. 1993, 7, 83-87.
• 8.Boryczko Z., Bostedt H., Hoffmann B.: Comparison of the hormonal and chemical composition of the fluid from bovine ovarian follicles and cysts. Reprod. Dom. Anim. 1995, 30, 36-38.
• 9.Calder M. D., Manikkam M., Salfen B. E., Youngquist R. S., Lubahn D. B., Lamberson W. R., Garverick H. A.: Dominant bovine ovarian follicular cysts express increased levels of messenger RNAs for luteinizing hormone receptor and 3 beta-hydroxysteroid dehydrogenase delta (4), delta (5) isomerase compared to normal dominant follicles. Biol. Reprod. 2001, 65, 471-476.
• 10.Donesky B. W., Adashi E. Y.: Surgically induced ovulation in the polycystic ovary syndrome: wedge resection revisited in the age of laparoscopy. Fertil. Steril. 1995, 63, 439-463.
• 11.Dorfman M., Arancibia S., Fiedler J. L., Lara H. E.: Chronic intermittent cold stress activates ovarian sympathetic nerves and modifies ovarian follicular development in the rat. Biol. Reprod. 2003, 68, 2038-2043.
• 12.Dynarowicz I., Dziegielewski M.: The role of the cholinergic system in the regulation of blood flow through the reproductive organs of swine during the estrous cycle. Pol. Arch. Vet. 1987, 27, 69-83.
• 13.Dzienis A., Majewski M., Wojtkiewicz J., Jana B.: Changes in density of sympathetic nerve terminals and steroidogenic activity of porcine ovaries after dexamethasone-induced polycystic ovarian syndrome. Pol. J. Vet. Sci. 2004, 7, 37-39.
• 14.Dzienis A., Majewski M., Wojtkiewicz J., Piskuła M., Jana B.: Adrenergic innervation and steroidogenic activity of cystic porcine ovaries. Rocz. Akademii Medycznej w Białymstoku 2004, 49, 114-116.
• 15.Frautschy S. A., Liptrap R. M.: Anovulation and plasma hormone concentrations after administration of dexamethasone during the middle of the luteal phase in sows undergoing estrous cycles. Am. J. Vet. Res. 1988, 49, 1270-1275.
• 16.Hong D., Byers M. R., Oswald R. J.: Dexamethasone treatment reduces sensory neuropeptides and nerve sprouting reactions in injured teeth. Pain 1993, 55, 171-181.
• 17.Jana B., Dzienis A., Rogozińska A., Piskuła M., Jedlinska-Krakowska M., Wojtkiewicz J., Majewski M.: Dexamethasone-induced changes in sympathetic innervation of porcine ovaries and in their steroidogenic activity. J. Reprod. Dev. 2005, 6, 715-725.
• 18.Jorgensen J. C., Kannisto P., Liedberg F., Ottesen B., Owman C., Schmidt G.: The influence of neuropeptide Y and norepinephrine on ovulation in the rat ovary. Peptides 1991, 12, 975-982.
• 19.Lakomy M.: Unerwienie adrenergiczne i cholinergiczne narządów układu rozrodczego samiczego świni w różnych okresach cyklu jajnikowego, ciąży i laktacji. Zesz. Prob. Post. Nauk Roln. 1987, 339, 89-104.
• 20.Lara H. E., Ferruz J. L., Luza S., Bustamante D. A., Borges Y., Ojeda S. R.: Activation of ovarian sympathetic nerves in polycystic ovary syndrome. Endocrinology 1993, 133, 2690-2695.
• 21.Majewski M.: Afferentne i efferentne unerwienie jajnika świni - źródła pochodzenia i kodowanie chemiczne. Praca hab., Wyd. ART, Olsztyn 1997.
• 22.Majewski M., Heym C.: The origin of ovarian neuropeptide Y (NPY)-immunoreactive nerve fibres from the inferior mesenteric ganglion in the pig. Cell Tissue Res. 1991, 266, 591-596.
• 23.Markiewicz W., Jaroszewski J. J., Bossowska A., Majewski M.: NPY: its occurrence and relevance in the female reproductive system. Folia Histochem. 2003, 41, 183-192.
• 24.Masuda M., Kubota T., Aso T.: Effects of nitric oxide on steroidogenesis in porcine granulose cells during different stages of follicular development. Eur. J. Endocrinol. 2001, 3, 303-308.
• 25.Nakamura Y.: Treatment of polycystic ovary syndrome: an overview. Horm. Res. 1990, 33, 31.
• 26.Ogasa A., Domeki I., Itoh S., Tsutsui T., Kawakami E.: The formation of cystic ovarian follicles in the sows after treatment with ACTH. Jap. J. Zootech. Sci. 1988, 59, 1004-1012.
• 27.Paredes A., Galvez A., Leyton V., Aravena G., Fiedler J. L., Bustamante D., Lara H. E.: Stress promotes development of ovarian cysts in rats: the possible role of sympathetic nerve activation. Endocrine 1998, 8, 309-315.
• 28.Parra C., Fiedler J. L., Luna S. L., Greiner M., Padmanabhan V., Lara H. E.: Participation of vasoactive intestinal polypeptide in ovarian steroids production during the rat estrous cycle and in the development of estradiol valerate-induced polycystic ovary. Reproduction 2007, 133, 147-154.
• 29.Pitzel L., Jarry H., Wuttke W.: Effects of substance-P and Neuropeptide-Y on in vitro steroid release by porcine granulosa and luteal cells. Endocrinology 1991, 129, 1059-1065.
• 30.Ryan P. L., Raeside J. I.: Cystic ovarian degeneration in pigs: a review. Second of two parts. Irih Vet. J. 1991, 44, 27-36.
• 31.Stones R. W., Loesch A., Beard R. W., Burnstock G.: Substance P: endothelial localization and pharmacology in the human ovarian vein. Obstet. Gynecol. 1995, 85, 273-278.
• 32.Trzeciak W. H., Ahmed C. E., Simpson E. R., Ojeda S. R.: Vasoactive intestinal peptide induces the synthesis of the cholesterol side-chain cleavage enzyme complex in cultured rat ovarian granulosa cells. Proc. Natl. Acad. Sci. 1986, 83, 7490-7494.
• 33.Viveiros M. M., Liptrap R. M.: Altered ovarian follicle function in ACTH-treated gilts. Anim. Reprod. Sci. 1995, 40, 107-119.
• 34.Wulff C., Wilson H., Wiegand S. J., Rudge J. S., Fraser H. M.: Prevention of thecal angiogenesis, antral follicular growth, and ovulation in the primate by treatment with vascular endothelial growth factor trap R1R2. Endocrinology 2002, 143, 2797-2807.