Growth inhibition of Fusarium verticillioides [Sacc.] Nirenberg by isolates of Trichoderma pseudokoningii strains from maize plant parts and its rhizosphere

• Autorzy: Sobowale A A , Cardwell K.F. , Odebode A.C. , Bandyopadhyay R. , Jonathan S.G.

Warianty tytułu

PL

Hamowanie wzrostu Fusarium verticillioides [Sacc.] Nirenberg przez szczepy Trichoderma pseudokoningii izolowane z roslin kukurydzy oraz rizosfery

• Języki publikacji: EN

Abstrakty

EN

Ability of five strains of Trichoderma pseudokoningii (antagonists) to suppress radial growth of Fusarium verticillioides (Sacc.) Nirenberg (=Fusarium moniliforme Sheldon) was examined i n vi tro. These were T. pseudokoningii strain1 (IMI 380933), strain2 (IMI 380937), strain3 (IMI 3809 39), strain4 (IMI 380940) and strain5 (IMI 380941). Each strain was paired with pathogen by inoculating at opposite ends of 9 cm petri plates using three pairing methods. Gradings were assigned to varied growth inhibition of pathogen by antagonists and analysed using GLM procedure (SAS). Growth suppressionof F. verticillioides by all strains of T. pseudokoningii was significantly different (R2 =0.98, p=0.05) from control in all pairing methods. It differed significantly (p>0.0003) among the strains in all pairing methods. Growth suppression also differed significantly among (p>0.0001) and within (p>0.018) pairing methods. Growth suppression was best when antagonists were inoculated before pathogen. Suppression mechanisms include mycoparasitism and competition for space and nutrients. T. pseudokoningii strains 3 and 4 had the best (p=0.05) growth suppressionof F. verticillioides and could be used as biocontrol agents for endophytic F. verticillioides in maize plant. This experiment was conducted in the search for resident microorganisms that might be capable of checking F. verticillioides withinmaize plant by competitive exclusion in subsequent experiments.

PL

W warunkach in vitro zbadano aktywność pięciu szczepów Trichoderma pseudokoningii w hamowaniu wzrostu fitopatogennego szczepu Fusarium verticillioides – sprawcy fuzaryjnego uwiądu kukurydzy. Każdy szczep antagonisty wysiewano w szalkach Petriego z grzybem chorobotwórczym i analizowano ich wzrost zgodnie z metodyką GLM(SAS). Najsilniejsze hamowanie wzrostu F. verticillioides stwierdzono, gdy antagonista był inokulowany jako pierwszy. Szczep nr 3 T. pseudokoningii wykazał najsilniejsze działanie i może być przydatny w biologicznej ochronie kukurydzy przed fuzaryjnym uwiądem.

Słowa kluczowe

EN

plant protection; growth inhibition; Fusarium verticillioides; isolate; Trichoderma pseudokoningii; maize plant; plant part; rhizosphere; antagonist; pathogen; persistence; internode; fungal pathogen

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2005
• Tom: 45
• Numer: 4
• Opis fizyczny: p.249-265,fig.,ref.

Twórcy

autor

Sobowale A A

• Olabisi Onabanjo University, PMB 2002, Ago-Iwoye, Ogun State, Nigeria

autor

Cardwell K.F.

autor

Odebode A.C.

autor

Bandyopadhyay R.

autor

Jonathan S.G.

Bibliografia

• Ahmed A.S., Sánchez C., Candela E.M. 2000. Evaluation of induction of systemic resistance inpepper plants (Capsicum annuum) of Phytophthora capsici using Trichoderma harzianum and its relation with capsidiol accumulation. Eur. J. Plant Pathol., 106: 817-824.
• Bunting E.S., Pain B.F., Phipps R.H., Wilkinson J.M., Gunn R.E. 1978. Forage Maize: Production and Utilization. Agricultural Research Council, London, 346 pp.
• Campbell R.B. 1988. Biological Control of Microbial Plant Pathogens. Cambridge Univ. Press, Cambridge, 218 pp.
• DeaconJ.W., Berry L.A. 1992. Modes of actionof mycoparasites inrelationto biocontrol of soilborne plant pathogens. p. 157–165. In “Biological Control of Plant Diseases” (E.S. Tjamos, G.C. Papaviza, R.J. Cook, eds.). Plenum Press, New York.
• Dhingra O.D., Sinclair J.B. 1985. Soil microorganisms. p. 179–221. In “Basic Plant Pathology Methods”. CRC Press, Inc., Boca Raton, FL.
• EtebarianH.R., Scott E.S., Wicks T.J. 2000. Trichoderma harzianum T39 and T. virens DAR 74290 as potential biological control agents for Phytophthora erythroseptica. Eur. J. Plant Pathol., 106: 329–337.
• Hayes C.K. 1992. Improvement of Trichoderma and Gliocladium by Genetic Manipulation. p. 277–286. In “Biological Control of Plant Diseases” (E.C. Tjamos, G.C. Papavizas, R.J. Cook, eds.). Progress and Challenges for the Future. Plenum Press, New York.
• Howell C.R. 2003. Mechanism employed by Trichoderma species inthe biological control of plant diseases: The history and evolution of current concepts. Plant Dis., 87: 4 –10.
• Janisiewicz W.J. 1988. Biocontrol of postharvest diseases of apples with antagonistic mixtures. Phytopathology 78: 194–198.
• Julian A.M. Wareing P.W., Philips S.I., Medlock V.F.P., MacDonald M.V., Del Rio L.E. 1995.
• Fungal contamination and selected mycotoxins in pre- and postharvest maize in Honduras. Mycopathologia 129: 5–16.
• MacDonald M.V., Chapman R. 1996. The incidence of Fusarium moniliforme onmaize from Central America, Africa and Asia during 1992–1995. Plant Pathol., 46: 112–125.
• Marasas W.F.O. 1988. Medical relevance of mycotoxins in Southern Africa. Microbiol. Aliments Nutrition 6: 1–5.
• McKeen W.E. 1953. Preliminary studies of root and basal stalk rot of maturing corn in Ontario. Can. J. Bot., 31: 132–141.
• Munkvold G.P., Carlton W.M. 1996. Influence of inoculation method on systemic Fusarium moniliforme infection of maize plants grown from infected seeds. Plant Dis., 81: 211–216.
• NelsonP.E., ToussounT.A., Marasas W.F.O. 1983. Fusarium species, AnIllustrated Manual for Identification. Pennsylvania State University, University Park.
• Paavanen-Huhtala S., Avikainen H., Yli-Mattila T. 2000. Development of strain-specific primers for a strainof Gliocladium catenulatum used in biological control. Eur. J. Plant Pathol., 106:187–198.
• Roane C.M. 1950. Observations on corn diseases in Virginia from 1947–1950. Plant Dis. Reporter 34: 394–396.
• Roberts R.G. 1990. Postharvest biological control of gray mold of apple by Cryptococcus laurentii. Phyptopathology 80: 526-530.
• Sharma J.K., Sankaran K.V. 1988. Biocontrol of rust and leaf spot diseases. KFRI Scientific Paper No. 133. p. 1–23. In “Biocontrol of Plant Diseases” (K.J. Mukerji, K.L. Gary, eds.). CRC Press, Boca Raton, F. L. Vol. II.
• Smith D., Onions A.H.S. 1983. The comparison of some preservation techniques for fungi. Trans. Br. Mycol. Soc., 81: 535–540.
• Tuite J. 1969. Isolation of bacteriophage and plant pathogenic actinomycetes, bacteria and fungi. p. 92–111. In “Plant Pathological Methods. Fungi and Bacteria”. Burgess Publishing Co., Minneapolis.
• Visconti A., Doko M. 1994. Survey of fumonisin production by Fusarium isolated from cereals in Europe. J. AOAC Intern., 77: 546–550.
• Warcup J.H. 1950. The soil-plate method for isolationof fungi from soil. Nature 166: 117–118.
• Weindling R. 1932. Trichoderma lignorum as a parasite of other soil fungi. Phytopathology 22: 837–845.
• Wells H.D., 1988. Trichoderma as a biocontrol agent. p. 71–82. In “Biocontrol of Plant Diseases” (K.J. Mukerji, K.L. Gary, eds.). Vol. 1, CRC Press, Boca Raton, FL.
• Yates I.E., Meredith F., BaconC.W., Jaworski A.J. 2000. Fusarium moniliforme productionof fumonisin B1 suppressed by Trichoderma viride. J. Food Prot., 62: 1326–1332.