Solute diffusion through stripped mouse duodenum

• Autorzy: Takeuchi T. , Ham M. , Mizumori M. , Guth P.H. , Engel E. , Kaunitz J.D. , Akiba Y.
• Języki publikacji: EN

Abstrakty

EN

We measured villous cell intracellular pH (pHi) and solute diffusion between the bathing media and the epithelial cells in stripped, chambered mouse duodenum. Apical perfusion of a high CO2 solution rapidly acidified the upper villous cells with recovery after its removal. Apical zoniporide (ZP) enhanced CO2-induced acidification. Serosal ZP, dimethylamiloride (DMA) or stilbene anion transport inhibitors failed to alter CO2-induced acidification, whereas serosal high CO2 buffer acidified the upper villous cells. Serosal 5-hydroxytryptamine rapidly acidified the upper villous cells. All serosally-perfused fluorescent compounds stained the crypt area, but not the villi or villous cells. In contrast, intravenous carboxyfluorescein quickly diffused into the interstitial space of the entire mucosa, and mucosally perfused fluorescent compound rapidly penetrated the epithelial cell layer. In muscle-stripped duodenum mounted in a small-aperture perfusion chamber, serosal solutes can readily diffuse only to the crypt cell region, whereas access to the villous epithelial cells is diffusion-limited. In contrast, rapid villous cell responses to serosally applied solutes are best explained by neural reflexes. Limited viability of the villous cells and impaired structural stability of the villi further limit long-term, villous cell functional studies of mucosal preparations mounted in small aperture diffusion chambers.

Słowa kluczowe

EN

diffusion barrier; upper villous cell; carbon dioxide; solute diffusion; mice; epithelial cell; 5-hydroxytryptamine; mouse; fluorescence; chemical diffusion; ion transport; dimethylamiloride; duodenum

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 4
• Opis fizyczny: p.767-791,fig.,ref.

Twórcy

autor

Takeuchi T.

• University of California Los Angeles, Los Angeles, CA USA

autor

Ham M.

autor

Mizumori M.

autor

Guth P.H.

autor

Engel E.

autor

Kaunitz J.D.

autor

Akiba Y.