Changes in DNA-binding activity of transcription factors in the differentiating bovine mammary gland

• Autorzy: Malewski T , Gajewska M. , Zwierzchowski L.
• Języki publikacji: EN

Abstrakty

EN

Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrophoretic mobility shift assay (EMSA) of nuclear proteins derived from bovine mammary glands showed for the first time the presence there of CREB, NF-κB, Oct1 and Sp1 transcription factors. The pattern of DNA-protein complexes of Sp1 transcription factor significantly differed between virgin heifers and lactating cows. Transition from pregnancy to lactation was associated with changes in the DNAprotein binding patterns of NFI and Oct1 transcription factors.

PL

Wcześniejsze badania autorów prowadzone in silico wykazały obecność potencjalnych miejsc wiązania dla wielu czynników transkrypcyjnych w rejonach 5’-flankujących genów białek mleka. W obecnej pracy przedstawiono wyniki analizy poszerzonej o doświadczalne badanie wiązania tych czynników transkrypcyjnych z DNA. Stosując analizę EMSA z użyciem białek jądrowych gruczołu mlekowego bydła w różnych stadiach jego rozwoju, po raz pierwszy wykazano obecność w nim czynników transkrypcyjnych CREB, NF-κB, Oct1 i Sp1. Kompleksy DNA-białko czynnika transkrypcyjnego Sp1 wykazały różne wzory ruchliwości elektroforetycznej u dziewiczych i cielnych jałówek. Przejście od ciąży do laktacji wiązało się z dalszymi zmianami w kompleksach DNA-białko czynników transkrypcyjnych NFI i Oct1.

Słowa kluczowe

EN

transcription factor; cattle; bovine mammary gland; milk protein; animal physiology; DNA binding; animal breeding; mammary gland; casein gene

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2005
• Tom: 23
• Numer: 2
• Opis fizyczny: p.75-84,fig.,ref.

Twórcy

autor

Malewski T

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Gajewska M.

autor

Zwierzchowski L.

Bibliografia

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