Wplyw nowo zsyntetyzowanych seskwiterpenowych analogow taksolu na namnazanie wirusa opryszczki [HSV-1 MC] oraz retrowirusa [Mo-MSV]

• Autorzy: Kobus M. , Krawczyk E. , Kopczacki P. , Daniewski W. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

Zbadano 9 nowo zsyntetyzowanych N-benzoilofenyloserynianów seskwiterpenów o budowie analogicznej do taksolu w kierunku ich przeciwwirusowej aktywności. Badania przeprowadzono w układzie in vitro na modelu zakażeń hodowli komórkowej Vero wirusem HSV-1Mc oraz w układzie in vivo, badając wpływ związków na przebieg zakażenia myszy retrowirusem Mo- MSV.

EN

The study comprised newly synthesized sesquiterpenoid analogs of taxol. The synthesis of the compounds was performed at the Institute of Organic Chemistry, Polish Academy of Sciences. Cytotoxicity of the compound was assessed using formazan method. In in vitro studies the cell cultures were infected with HSV-1Mc. The tested compounds were added in different concentrations to the cell culture after viral infection. Titer of the virus was expressed in TCIDW50/ml at particular stages of the experiments. In in vivo experiments NMRI mice were infected intramuscularly with a Moloney murine sarcoma virus (Mo-MSV). Tested compounds were administered to the mice intravenously on the day of virus inoculation. In Mo-MSV-infected mice dynamics of tumor progression and regression was assessed, as well as a mean time interval of tumor disappearance. Among the compounds tested: isovellerol-13-N-benzoyl-(2'R,3'S)-3'-phenylisoserinate, 5-deoxy-lactarolid B 8-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] and isolactarorufin 8-epi-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] showed significant antiviral activity in in vitro experiments. In in vivo experiments only lactarorufin A 8-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] significantly inhibited the development of tumors and shortened the time of their total regression in the course of Mo-MSV infection.

Słowa kluczowe

PL

N-benzoilofenyloseryniany seskwiterpenow; retrowirusy; infekcja wirusowa; aktywnosc przeciwwirusowa; wirusy; mikrobiologia lekarska; komorki; taksol; opryszczka; leki; hodowla in vitro

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2001
• Tom: 53
• Numer: 3
• Opis fizyczny: s.283-289,rys.,wykr.,bibliogr.

Twórcy

autor

Kobus M.

• Akademia Medyczna, Warszawa

autor

Krawczyk E.

autor

Kopczacki P.

autor

Daniewski W.

autor

Luczak M.

Bibliografia

• 1. Daniewski W, Vidari G. Constituents of Lactarius (Mushrooms). Progress in the Chemistry of Organic Natural Products. SpringerWienNewYork, 1999.
• 2. Denyer CV, Jackson P, Loakes DM i inni. Isolation of antirhinoviral sesquiterpenes from ginger (Zingiber officinale). J Nat Prod 1994; 57: 658-62.
• 3. De Tommasi N, Pizza C, Conti C i inni. Structure and in vitro antiviral activity of sesquiterpene glycosides from Calendula arvensis. J Nat Prod 1990; 53: 830-35.
• 4. Dunnet CW. Multiple comparison procedure for comparing several treatments with a control. J Am Stat Assoc 1955; 50: 1095-1121.
• 5. Hayashi K, Hayashi T, Ujita K, Takaishi Y. Characterization of antiviral activity of a sesquiterpene, triptofordin C-2. J Antimicrob Chemoth 1996; 37: 759-68.
• 6. Kobus M, Łuczak M, Krawczyk E i inni. Effect of 5,10a, 13-trihydroxy-2a, 9a-H-marasm-7(8)- en on experimental Herpes simplex virus type 1 infections (in vitro and in vivo). Int Proc Div 2001 - w druku.
• 7. Simon B, Anke T, Anders U i inni. Collybial, a new antibiotic sesquiterpenoid from Collybia confluens (Basidiomycetes). Z Naturforsch C 1995; 50: 173-80.
• 8. Sotanaphun U, Lipipun V, Suttisri R, Bavovada R. A new antiviral and antimicrobial sesquiterpene from Glyptopetalum sclerocarpum. Planta Med 1999; 65: 257-58.
• 9. Włodarski K, Kobus M, Łuczak M. Orthotopic bone induction at sites of Moloney murine sarcoma virus inoculation in mice. Nature 1979; 281: 386-87. 10. Wright AE, Rueth SA, Cross SS. An antiviral sesquiterpene hydroquinone from the marine sponge Strongylophora hartmani. J Nat Prod 1991; 54: 1108-11.