In vitro culture of unfertilized ovules of Viola odorata L.

• Autorzy: Wijowska M. , Kuta E. , Przywara L.
• Języki publikacji: EN

Abstrakty

EN

Callus induction, organogenesis, and autonomous endosperm were induced by in vitro culture of unfertilized ovules of six genotypes of Viola odorata L. (2n = 20). On media supplemented with auxins and cytokinins the ovules enlarged; the frequency of enlarged ovules depended on plant genotype, the stage of ovule development and the hormones used. The highest frequency of enlarged ovules was on MS medium with 2,4-D and kinetin. Autonomous endosperm was observed in ovules after 6 days of culture on MS medium with 0.1 mg l-1 2,4-D. No egg cell divisions were found in examined ovules. The highest rate of callus induction was on MS medium with 2,4-D and BAP. Roots regenerated from callus on the same medium and on media with different concentrations of auxin and cytokinin. Karyological variability of callus cells and regenerated roots was observed, with euploid and aneuploid chromosome numbers in roots (2n = 13, 15, 17, 18, 20) and diploid and polyploid in the callus (2n = 20, 30, 40). The origin of callus is discussed.

Słowa kluczowe

EN

embryo sac; callus; chromosome number; autonomous endosperm; organogenesis; unfertilized ovule; in vitro; Viola odorata

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1999
• Tom: 41
• Opis fizyczny: p.95-101,fig.

Twórcy

autor

Wijowska M.

• Jagiellonian University, Grodzka 52, 31-044 Cracow, Poland

autor

Kuta E.

autor

Przywara L.

Bibliografia

• Banasińska A, and Kuta E. 1996. Allogamy in Viola odorata L. Acta Biologica Cracoviensia Series Botanica 38: 42-51.
• Bohanec B. 1994. Induction of gynogenesis in agricultural crops: a review. Proceedings of IPBA, 5-7 December 1994, 43-55, Rogla.
• Chmielowiec M, Kuta E, Przywara L, and Róg L. 1997. An attempt to induce gynogenesis in Brassica napus L. Acta Biologica Cracoviensia Series Botanica et Zoologia 39, suppl. 1: 49.
• Huang QF, Yang H, and Zhou C. 1982. Embryological observations on ovary culture of unpollinated young flowers of Hordeum vulgare. Acta Botánica Sinica 24: 295-300.
• Jensen WA, Schultz P, and Ashton ME. 1977. An ultrastructural study of early endosperm development and synergid changes in unfertilized cotton ovules. Planta 133: 179-189.
• Mukhambetzhanov SK. 1997. Culture of unfertilized female gametophytes in vitro. Plant Cell, Tissue and Organ Culture 48: 111-119.
• Mól R, Betka A, and Wojciechowicz M. 1995. Induction of autonomous endosperm in Lupinas luteus, Helleborus niger and Melandrium album by in vitro culture of unpollinated ovaries. Sexual Plant Reproduction 8: 273-277.
• Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473-497.
• Yang HY, and Zhou C. 1982. In vitro induction of haploid plants from unpollinated ovaries and ovules. Theoretical and Applied Genetics 63: 97-104.
• Yang H, Zhou C, Cai D, Yan H, Wu Y, and Chen X. 1986. In vitro culture of unfertilized ovules in Helianthus annuus L. In: Hu H and Yang H [eds.], Haploids of higher plants in vitro, 182-191. China Academic Publishers Beijing. Springer-Verlag, Berlin, Heidelberg, New York, Tokyo.
• Zhou C, and Yang HY. 1980. In vitro induction of haploid plantlets from unpollinated young ovaries of Oryza sativa L. Acta Genetica Sinica 7: 287-288.
• Zhou C, and Yang HY. 1981a. Studies on the in vitro induction of callus from embryo sacs of rice. Hereditas (Beijing) 3: 10-12.
• Zhou C, and Yang HY. 1981b. Induction of haploid rice plantlets by ovary culture. Plant Science Letters 20: 231-237.