Blood plasma antioxidants and dehydration in three-month-long trained Standardbred trotters before and after an intensive race

• Autorzy: Ogonski T , Pikula R. , Majewski G.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine the total antioxidant status in the plasma of horses - Standardbred trotters, exposed to extreme oxygen action during a race. The experiment was carried out on 10 two-year old horses - five stallions and five mares, after three-month-long initial training. On the experimental day, peripheral blood was sampled twice from the horses: at rest and after a race over 3200 m at a pace of 550 m/min. In the blood were assayed: blood platelet, white blood cell and red blood cell counts as well as haematocrit value and haemoglobin concentration. The obtained values were the basis for calculating plasma dehydration factor (Df). In the plasma was determined the antioxidative ability of plasma (AAP). For assays, a TAS (Total Antioxidant Status) analytical kit of Randox manufacture (UK) was used. In 8/10 horses, the AAP value underwent a reduction after the race. The statistical analysis revealed a significant decrease in AAP value in horses after completing the race (Pp = 0.0126). This difference correlates with the value of Df factor (R² = 0.304), which may mean that prolonged organism overloading with intensive oxygen work leads to progressive exhaustion of its antioxidative reserves and in consequence, after crossing the critical value, to development of oxygen stress. The largest decrease of AAP value occurred in the horses capable of performing the largest effort (ΔTAS = 0.20 ± 0.14 mol/dm³). These animals, i.e. three stallions and three mares, were characterised by a lower heartbeat rate and a lower respiration frequency at rest as well as by the capacity for quick regeneration of these parameters after the completed race.

Słowa kluczowe

EN

dehydration; intensive race; Standardbred trotter; race exercise; horse; blood plasma antioxidant; race adaptation degree; blood morphology; trotter; training

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 04A
• Opis fizyczny: p.421-426,fig.,ref.

Twórcy

autor

Ogonski T

• University of Agriculture, Doktora Judyma 2, 70-466 Szczecin, Poland

autor

Pikula R.

autor

Majewski G.

Bibliografia

• 1.Arnason T., Darenius A., Philipsson J.: Genetic selection indices for Swedish trotter broodmares. Livest. Prod. Sci. 1982, 8, 557-565.
• 2.Avellini L., Chiaradia E., Gati A.: Effect of exercise training. selenium and vitamin E on some free radical scavengers in horses (Equus caballus). Comp. Biochem. Physiol. B 1999, 123, 147-152.
• 3.Balogh N., Gaal T., Ribiczeyne P. S., Petri A.: Biochemical and antioxidant changes in plasma and erythrocytes of pentathlon horses before and after exercise. Vet. Clin. Pathol. 2001, 30, 214-218.
• 4.Bis-Wencel H., Saba L., Odój J., Nowakowicz-Dębek B., Kaproń B.: Wybrane wskaźniki stanu oksydacyjnego koni przed okresem intensywnego użytkowania rekreacyjnego i w trakcie jego trwania. Medycyna Wet. 2002, 58, 992-994.
• 5.Frankiewicz-Jóźko A., Szarska E.: Ocena stanu antyoksydacyjnego we krwi a zdolność do wysiłku koni. Życie Wet. 2001, 76, 156-158.
• 6.Hellsten Y., Svensson M., Sjodin B., Smith S., Christensen A., Richter E. A., Bangsbo J.: Allantoin formation and urate and glutathione exchange in human muscle during submaximal exercise. Free Radic. Biol. Med. 2001, 27, 146-159.
• 7.Jenkins R. R., Friedland R., Howald H.: The relationship of oxygen uptake to superoxide dismutase and catalase activity in human skeletal muscle. Int. J. Sports Med. 1984, 57, 86-92.
• 8.Ji L. L.: Antioxidants and oxidative stress in exercise. Proc. Soc. Exp. Biol. Med. 1999, 222, 283-292.
• 9.Katona O.: Genetical-statistical analysis of traits in the German trotter. Livest. Prod. Sci. 1979, 6, 407-412.
• 10.Kędzierski W., Podolak M.: Zmiany metaboliczne u koni w procesie ujeżdżania. Medycyna Wet. 2001, 57, 207-209.
• 11.Leeuvenburgh C., Heinecke J. W.: Oxidative stress and antioxidants in exercise. Curr. Med. Chem. 2001, 8, 829-838.
• 12.Marlin D. J., Fenn K., Smith N., Deaton C. D., Roberts C. A., Harris P. A., Dunster C., Kelly F. J.: Changes in circulatory antioxidant status in horses during prolonged exercise. J. Nutr. 2002, 132, 1622S-1627S.
• 13.Mills P. C., Smith N. C., Casas J., Harris P., Harris R. C., Marlin D. J.: Effects of exercise intensity and environmental stress on indices of oxidative stress and iron homeostasis during exercise in the horse. Eur. J. Appl. Physiol. Occup. Physiol. 1996, 74, 60-66.
• 14.Minkema D.: Studies on the genetics of trotting performance in Dutch trotters. Ann. Genet. Sel. Anim. 1975, 7, 99-121.
• 15.Podmore I. D., Griffiths H. R., Herbert K. E., Mistry N., Mistry P., Lunec J.: Vitamin C exhibits pro-oxidant properties. Nature 1998, 392, 559.
• 16.Sitarska E., Kleczkowski M., Kluciński W., Ładysz R., Górecka R., Dziekan P.: Stan antyoksydacyjny koni i próba modulacji tego układu. Medycyna Wet. 1997, 53, 585-588.
• 17.Szarska E.: Badania laboratoryjne w treningu koni. Wyd. Agencja Reklamowa CREX s.c. Warszawa 2000.
• 18.Szarska E.: Ocena charakteru wysiłku koni skokowych w zależności od wielkości obciążenia. Życie Wet. 2002, 77, 36-38.
• 19.Szarska E.: Ocena wydolności koni podczas zawodów konkurencji WKKW. Medycyna Wet. 1994, 50, 274-276.
• 20.Szarska E.: Wykorzystanie badań diagnostycznych krwi do oceny stanu zdrowia i zaawansowania treningowego koni wyczynowych. SGGW, Warszawa 2002.
• 21.Szarska E.: Zmiany wybranych parametrów krwi koni rajdowych w zależności od długości dystansu. Medycyna Wet. 2001, 57, 522-526.
• 22.Viña J., Gomez-Cabrera M. C., Lloret A., Marquez R., Minana J. B., Pallardo F. V., Sastre J.: Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB Life 2000, 50, 271-277.