Cytokinin oxidase-dehydrogenase [CKX] activity in wild and ethylene-insensitive mutant eti5 type of Arabidopsis thaliana [L.] Heynh plants and the effect of cytokinin N1-[2-chloro-4-pyridyl]-N3-phenylurea on enzymatic activity and leaf morphology

• Autorzy: Todorova D , Vaseva-Gemisheva I. , Petrov P. , Stoynova-Bakalova E. , Alexieva V. , Karanov E. , Smith A. , Hall M.
• Języki publikacji: EN

Abstrakty

EN

The specific activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) (CKX) was determined in leaves of wild type (wt) and ethylene-insensitive mutant (eti5) of Arabidopsis thaliana (L.) Heynh plants. Comparative studies showed that this mutation has lower basal CKX activity than wt. Application of 4PU-30 (N1-(2-chloro-4-pyridyl)-N2-phenylurea) resulted in decreased CKX activity in both wt and mutant plants. The treatment increased leaf blade thickness and the volume of chlorophyll-containing cells per unit leaf area in wt but these changes were not observed in the eti5 mutant. The reduction in chlorophyll “a” and “b”, as well as in carotenoids content in the treated wt tissues resulting from altered leaf morphology was not detected in eti5 plants.

Słowa kluczowe

EN

plant physiology; ethylene-insensitive mutant; Arabidopsis thaliana; enzyme activity; eti5 type mutant; morphology; leaf; N1-[2-chloro-4-pyridyl]-N3-phenylurea; cytokinin oxidase-dehydrogenase

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2006
• Tom: 28
• Numer: 6
• Opis fizyczny: p.613-617,fig.,ref.

Twórcy

autor

Todorova D

• Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

autor

Vaseva-Gemisheva I.

autor

Petrov P.

autor

Stoynova-Bakalova E.

autor

Alexieva V.

autor

Karanov E.

autor

Smith A.

autor

Hall M.

Bibliografia

• Arnon D. 1949. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol., 24: 1-15.
• Bilyeu K. D., Cole J.L., Laskey J.G., Riekhof W.R., Esparza T.J., Kramer M.D., Morris R.O. 2001. Molecular and biochemical characterisation of a cytokinin oxidase from maize. Plant Physiol., 125: 378-386.
• Bradford M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
• Burch L. R., Horgan R. 1989. The purification of cytokinin oxidase from Zea mays kernels. Phytochem., 28: 1313-1319
• Gałuszka P., Frébort I., Šebela M., Sauer P., Jacobsen S., Pec P. 2001. Cytokinin oxidase or dehydrogenase? Eur. J. Biochem., 268: 450-461.
• Gan S., Amasino R. M. 1995. Inhibition of leaf senescence by autoregulated production of cytokinin. Science, 270: 1986-1988.
• Hare P. D., van Staden J. 1994. Cytokinin oxidase: Biochemical features and physiological significance. Physiol. Plant., 91: 128-136.
• Harpham N., Berry A., Knee E., Roveda-Hoyos G., Raskin I., Sanders I., Smith A., Wood C., Hall M. 1991. The effect of ethylene on the growth and development of wild type and mutant Arabidopsis thaliana (L.) Heynh. Ann. Bot., 68: 55-62.
• Houba-Hérin N., Pethe C., d’Alayer J., Laloue M. 1999. Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts. Plant J., 17(6): 615-626.
• Kaminek M., Motyka V., Vankova R. 1997. Regulation of cytokinin content in plant cells. Physiol. Plant., 101: 689-700.
• Kudryakova N., Burkhanova E., Rakitin V., Yakovleva L., Smith A., Hall M., Kulaeva O. 2001. Ethylene and cytokinin in the control of senescence in detached leaves of Arabidopsis thaliana eti-5 mutant and wild-type plants. Russ. J. Plant Physiol., 48(5): 624-627.
• Laloue M., Fox J. E. 1989. Cytokinin oxidase from wheat. Plant Physiol., 90: 899-906.
• Letham D. S. 1971. Regulators of cell division in plant tissues. XII. A cytokinin bioassay using excised radish cotyledons. Physiol. Plant., 25: 391-396.
• Liberos-Minotta C. A., Tipton P. A. 1995. A colorimetric assay for cytokinin oxidase. Anal. Biochem., 231: 339-341.
• Lichtenthaler H. K., Buschmann C. 1978. Control of chloroplast development by red light, blue light and phytohormones. In: Chloroplast Development, ed. by G Akoyunoglou, J. H. Argyroudi-Akoyunoglou, Elsevier, Biomedical Press, Amsterdam: 801-816.
• Masferrer A., Arro M., Manzano D., Schaller H., Fernandez-Busquets X., Moncalean P., Fernandez B., Cunillera N., Boronat A., Ferrer A. 2002. Overexpression of Arabidopsis thaliana farnesyl diphosphate synthases (FPS1S) in transgenic Arabidopsis induces a cell death/senescence-like response and reduced cytokinin levels. Plant J., 30: 123-132.
• Redig P., Motyka V., van Onckelen H. A., Kaminek M. 1997. Regulation of cytokinin oxidase activity in tobacco callus expressing the T-DNA ipt gene. Physiol. Plant., 99: 89-96.
• Sergiev I., Todorova D., Alexieva V., Karanov E., Smith A., Hall M. 2003. Rosette leaf senescence in wild type and ethylene-insensitive mutant of Arabidopsis thaliana (L.) Heynh inflorescence and fruit development. In: Phytohormones in Plant Biotechnology and Agriculture, ed. by I. Machachkova, G. Romanov, Kluwer Acad. Publ., Dordrech, The Nethelands: 217-228.
• Shudo K. 1994. Chemistry of phenylurea cytokinins. In: Cytokinins: Chemistry, Activity and Function, ed. by D. W. S. Mok, M. C. Mok, CRC Press, Boca Raton: 35-42.
• Spurr A. R. 1969. A low-viscosity epoxy resin embedding medium for election microscopy. J. Ultrastruct. Res., 26: 31-43.
• Todorova D., Alexieva V., Karanov E. 2002. Polyamine levels in Arabidopsis thaliana (L.) Heynh, plants during their development. Compt. rend. Acad. Bulg. Sci., 55(4): 93-96.
• Todorova D., Genkov T., Vaseva-Gemisheva I., Alexieva V., Karanov E., Smith A., Hall M. 2005. Effect of temperature stress on the endogenous cytokinin content in Arabidopsis thaliana (L.) Heynh plants. Acta Physiol. Plant., 27(1): 13-18.
• Vaseva-Gemisheva I., Sergiev I., Todorova D., Alexieva V., Stanoeva E., Lachkova V., Karanov E. 2005. Antagonistic effects oftriazolo[4, 5-d]pyrimidine and pyridylurea derivatives on cytokinin-induced cytokinin oxidase/dehydrogenase activity in young pea plants. Plant Growth Regul., 46(3): 193-197.
• Wingler A., van Schäwen A., Leegood R. C., Lea P. J., Quick W. P. 1998. Regulation of leaf senescence by cytokinin, sugars, and light. Plant Physiol., 116: 329-335.