Opracowanie zestawu diagnostycznego do wykrywania przeciwcial anty-Hypoderma bovis u bydla testem ELISA. 1.Uzyskanie komponentow

• Autorzy: Ziomko I , Cencek T.
• Języki publikacji: PL

Abstrakty

EN

The aim of these investigations was to prepare the antigen and control sera for the ELISA. Positive control sera were obtained from 163 cows showing clinical symptoms of H. bovis infection and negative control sera from 60 cows free from the infection.The antigen for theELISA was obtained from H. bovis L1 larvae. Three basie fraction were isolated from whole excreted/secreted antigen by elution from gel and by preparative isoelectrofocusing.Using the Western-Blotting method it was demonstrated that Hy A (22-24 kDa and pI 4-5) had the best antigenic property. As can be seen from these investigations the developed ELISA kit for the detection of H. bovis antibodies in cows is highly specific and sensitive.

Słowa kluczowe

PL

pasozyty zwierzat; bydlo; choroby pasozytnicze; Hypoderma bovis; giez bydlecy; gzawica; diagnostyka; przeciwciala; parazytologia; przeciwciala anty-Hypoderma bovis; choroby zwierzat; wykrywanie; test ELISA

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 3
• Opis fizyczny: s.497-503,rys.,tab.,bibliogr.

Twórcy

autor

Ziomko I

• Panstwowy Instytut Weterynaryjny, ul.Partyzantow 57, 24-100 Pulawy

autor

Cencek T.

Bibliografia

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