Study on properties of enterohaemorrhagic Escherichia coli 0157 isolates and their survival under different conditions

• Autorzy: Kovalikova M , Pipova M. , Kantikova M. , Kozarova I. , Janosova J. , Martonova M. , Kovalik M.
• Języki publikacji: EN

Abstrakty

EN

During the period of 2004-2006, 955 samples of food and clinical material were collected from the Slovak Republic and Belgium. Of the total number of 216 food samples originating from the Slovakia territory, the authors obtained 5 isolates (2.31%) of Escherichia coli O157. Three E. coli O157 (2.30%) isolates were obtained during the examination of 130 samples of slaughter animals from the Belgium territory. In Slovakia no occurrence of E. coli O157 was proved in any sample of clinical material or environment (altogether 36 samples). On the contrary, of 573 clinical and environmental samples from Belgium the presence of the respective pathogen was proved in 50 cases (8.73%). The authors studied some attributes of the recovered E. coli O157, confirmed by immunomagnetic separation (IMS) and polymerase chain reaction (PCR). The results indicate that the isolates are capable of surviving for 15 min at 70°C. The acidic environment, which is characteristic for fermentation of cheese (pH 4-4.5), had no devitalisation effect. The E. coli food isolates survived in a wide range of pH (2-11) while with clinical isolates the pH range of survival was from 2.5 to 10.5. The addition of NaCl in concentrations ordinarily used in food industry (3-6%) failed to inhibit the growth of pathogens.

Słowa kluczowe

EN

temperature; isolate; clinical isolate; pathogen; food isolate; food sample; Slovak Republic; clinical material; Escherichia coli; pH; survival

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 05
• Opis fizyczny: p.654-657,fig.,ref.

Twórcy

autor

Kovalikova M

• University of Veterinary Medicine, Komenskeho 73, 040 01 Kosice, Slovak Republic

autor

Pipova M.

autor

Kantikova M.

autor

Kozarova I.

autor

Janosova J.

autor

Martonova M.

autor

Kovalik M.

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