Comparison of physiological and PCR-RFLP rDNA identification of yeast species commonly found in cheese

• Autorzy: Polomska X. , Juszczyk P. , Cadez N. , Raspor P. , Robak M. , Wojtatowicz M.

Warianty tytułu

PL

Porownanie identyfikacji gatunkow drozdzy powszechnie spotykanych w serach w oparciu o cechy fizjologiczne oraz PCR-RFLP rDNA

• Języki publikacji: EN

Abstrakty

EN

The identification of twenty four yeast isolates from cheese performed with API ID 32C simplified assimilation test and restriction fragments length polymorphism (PCR-RFLP) of ITS1-5.8S rDNA-ITS2 region was compared. The accordance of the results of both methods for eleven strains identified as Candida famata, five strains as C. lipolytica and four strains as C. sphaerica was observed. However, the other four yeast isolates classified with API as C. sphaerica revealed amplification products as well as restriction patterns characteristic for C. famata species.

PL

Porównano identyfikację dwudziestu czterech izolatów drożdży z serów wykonaną przy pomocy testu asymilacyjnego API ID 32C (tab. 1) oraz metodą polimorfizmu długości fragmentów restrykcyjnych (PCR-RFLP) regionu ITS1-5.8S rDNA-ITS2 (tab. 2). Zaobserwowano zgodność wyników otrzymanych obiema metodami w stosunku do jedenastu szczepów zidentyfikowanych jako Candida famata, pięciu jako C. lipolytica i czterech jako C. sphaerica. Jednakże pozostałe cztery izolaty drożdży, sklasyfikowane przez system API jako C. sphaerica wykazały produkty amplifikacji, jak również profile restrykcyjne charakterystyczne dla gatunku C. famata (rys. 1 i 2).

Słowa kluczowe

EN

yeast isolate; identification; Candida famata; Candida sphaerica; Candida lipolytica; cheese; assimilation test; restriction fragment length polymorphism; rDNA

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 2
• Opis fizyczny: p.221-226,fig.,ref.

Twórcy

autor

Polomska X.

• Agricultural University of Wroclaw, C.K.Norwida 25/27, 50-375 Wroclaw, Poland

autor

Juszczyk P.

autor

Cadez N.

autor

Raspor P.

autor

Robak M.

autor

Wojtatowicz M.

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