Low molecular mass products of depolymerization of purified mucin - attempts at isolation and characterization

• Autorzy: Minkiewicz I. , Gindzienski A.
• Języki publikacji: EN

Abstrakty

EN

Samples of crude mucin were incubated at room temperature for 48 and 96 h in a sodium azide containing buffer, pH 7.0. Then each sample was purified, reduced and alkylated with iodo[14C]acetamide. Electrophoretic analysis demonstrated that radioactivity was incorporated into the mucin subunits and proteins of 100 and 140 kDa. The results of our experiments suggest that the released proteins can be a part of mucin molecule, cleaved by proteolysis and reduction of disulfide bridges.

Słowa kluczowe

EN

subunit; mucin; isolation; autoradiography; electrophoresis; purified mucin; disulphide bridge; depolymerization

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 4
• Opis fizyczny: p.929-933,fig.

Twórcy

autor

Minkiewicz I.

• Medical Academy of Bialystok, A.Mickiewicza 2, 15-230 Bialystok, Poland

autor

Gindzienski A.

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