Ocena przydatnosci chromogennych podlozy Agar Candida ID 2 do identyfikacji grzybow rodzaju Candida

• Autorzy: Ciok-Pater E. , Zalas P. , Gospodarek E.

Warianty tytułu

EN

Evaluation of Agar Candida ID 2 [bioMerieux] a chromogenic medium for yeasts differentiation

• Języki publikacji: PL

Abstrakty

PL

Oceniając przydatność chromogennych podłoży Agar Candida ID 2 (CAN2) (bioMéricux) do identyfikacji grzybów Candidasp. użyto do badań 146 szczepów izolowanych od pacjentów. W celu porównania identyfikacji gatunków grzybów stosowano podłoże chromogenne CAN2, test API 20C AUX (bioMéricux) oraz test filamentacji. Stwierdzono zgodność rozpoznania gatunków za pomocą CAN2 oraz API 20C AUX dla 82,2% szczepów. Barwa kolonii różniła się w 17,8% przypadków od przedstawionej w tabeli identyfikacyjnej zaproponowanej przez producenta podłoża.

EN

Invasive diagnostic and therapeutic methods, widespread antibiotic therapy and rising percent of the immunocompromised patients cause incrementation of frequency of occurrence of the yeast infection. C. albicans is the most commonly isolated species of Candida from clinical samples. However, recently growth of frequency of isolation Candida non - albicans from clinical specimens have been observed. Yeast-like fungi different from C. albicans have become serious clinical problem. Conventional methods of identification of the yeast-like fungi carry away a lot time enough. Employment of chromogenie agar shortens latency on result. We decided to examine the usefulness of Agar Candida ID2 (CAN2) (bioMérieux) in the identification of Candida species. The subjects within the study were 146 of Candida spp. strains which were isolated from the clinical specimens of patients hospitalized at the University Hospital in Bydgoszcz. Germ tube test. Арі 20C AUX test (bioMérieux) and Agar Candida ID2 (bioMérieux) were used. We have ascertained correspondence of identifying species amounted to 82,2% of analyzed Candida species between API 20C AUX test and kind of growth on CAN2 medium. Divergence of results received between CAN2 medium and API 20C AUX test suggests necessity of conducting of verification data with other methods. In conclusion, our study shows that Agar Candida ID2 is an effective medium for the isolation yeast-like fungi and in preliminary identification of Candida species direct from clinical materials.

Słowa kluczowe

PL

podloze Agar Candida ID 2; bioMerieux zob.podloze Agar Candida ID 2; ocena przydatnosci; identyfikacja; grzyby; Candida; ludzie chorzy; zakazenia grzybicze; Candida albicans; Candida glabrata; Candida parapsilosis; Candida tropicalis; grzyby drozdzopodobne

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 3
• Opis fizyczny: s.247-251,rys.,bibliogr.

Twórcy

autor

Ciok-Pater E.

• Collegium Medicum im.L.Rydygiera w Bydgoszczy, Uniwersytet Mikolaja Kopernika w Toruniu, ul.M.Sklodowskiej-Curie 9, 85-094 Bydgoszcz

autor

Zalas P.

autor

Gospodarek E.

Bibliografia

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