Histochemical studies on Fasciola hepatica in mice. II. Oxidoreductase activity in experimentally induced fasciolosis

• Autorzy: Kolodziejczyk L
• Języki publikacji: EN

Abstrakty

EN

CFW strain white mice were infected with various doses (10, 30, 50 and 100) of Fasciola hepatica metacercariae administered per os. Using histochemical methods, the activity of selected oxidoreductases (α-glycerophosphate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, and cytochrome oxidase) was evaluated in the liver of the mice suffering from acute and chronic fasciolosis. Intensified anaerobic and aerobic respiration was recorded. The host’s compensatory mechanism observed in the form of intensified metabolism, was found to be related to the infection intensity.

Słowa kluczowe

EN

succinate dehydrogenase; mouse; alpha-glycerophosphate dehydrogenase; mice; isocitrate dehydrogenase; fasciolosis; Fasciola hepatica; enzyme activity; oxidoreductase activity; infection intensity; cytochrome oxidase

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1996
• Tom: 41
• Numer: 4
• Opis fizyczny: p.221-226,fig.

Twórcy

autor

Kolodziejczyk L

• Department of Biology and Medical Parasitology, Pomeranian Medical University, Al.Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland

Bibliografia

• Boczoń K., Wandurska E., Imiłkowska K. 1989. Mitochondrial ATP-ase as a measure of uncoupling of rat muscle mitochondria in experimental infection with Trichnella spiralis and Trichinella pseudospiralis. Wiadomości Parazytologiczne, 35, 435-448.
• Furmaga S. 1963. Histochemical investigations of the liver in the course of experimental fascioliasis in rabbits. Acta Parasitologica Polonica, 11,49-73.
• Galtier P., Eeckhoutte C., Larrien G. 1987. Fasciola hepatica: liver enzymes in rats and interaction with chemical inducers. Experimental Parasitology, 63, 189-194.
• Galtier P., Cambon-Gras C., Fernandez Y., Deltour P., Eeckhoutte C., Hoellinger H. 1994. Fasciola hepatica: liver microsomal membrane functions in host rat. Experimental Parasitology, 78, 175-182.
• Guttowa A. 1980. Kompensacyjne reakcje żywicieli. Wiadomości Parazytologiczne, 26, 355-357.
• Guttowa A. 1988. Homeostaza fizjologiczna organizmu żywicielskiego w układach pasożyt-żywiciel. Wiadomości Parazytologiczne, 34, 522-527.
• Hanish M. J. E., Topfer F., Lenton L. M., Behm C. A., Bygrave F. L. 1992. Restoration of mitochondrial energy-linked reactions following dexamethasone treatment of rats infected with the liver fluke Fasciola hepatica. Biochimica et Biophysica Acta, 1139, 196-202.
• Humiczewska M., Skotarczak B. 1986. Cyto- and histochemical studies on the enzymes activity in Balantidium coli (Malmsten) and Mesocricetus auratus (Waterhouse). II. Oxidoreductases. Acta Parasitologica Polonica, 30, 289-302.
• Kołodziejczyk L. 1994. Study on glycogen content in the liver of mouse vs. pathomorphological changes in experimental fasciolosis. Acta Parasitologica, 39, 211-216.
• Kołodziejczyk L. 1996. Histochemical studies on Fasciola hepatica in mice. I. Oxidoreductase activity in juvenile and adult liver flukes. Acta Parasitologica, 41, 214-220.
• Kołodziejczyk L., Gałka G. 1991. Aktywność dehydrogenazy bursztynianowej i oksydazy cytochromowej w homogenacie wątroby w przebiegu doświadczalnej fasciolozy. Materiały XVI Zjazdu PTP, 12-13 września 1991, Poznań, 126.
• Lenton L. M., Behm C. A., Bygrave F. L. 1994. Characterization of the oligomycin-sensitivity properties of the F₁F₀-ATPase in mitochondria from rats infected with the liver fluke Fasciola hepatica. Biochimica et Biophysica Acta, 1186, 237-242.
• Lenton L. M., Behm C. A., Bygrave F. L. 1995. Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition. Biochemical Journal, 307, 425-431.
• Maffei Facino R., Carini M., Genchi C., Tofanetti O., Casciarri I. 1989. Participation of lipid peroxidation in the loss of hepatic drug-metabolizing activities in experimental fascioliasis in the rat. Pharmacological Research, 21,549-560.
• Maffei Facino R., Carini M., Genchi C., Tofanetti O., Casciarri I., Bedoschi D. 1990. Antihepatotoxic properties of uridine- diphosphoglucose in liver fluke infection. Arzneimittelforschung, 40, 490-498.
• Niewiadomska K., Pojmańska T., Machnicka B., Grabda-Kazubska B. 1980. Zarys parazytologii ogólnej. PWN, Warszawa.
• Polyakova-Krusteva O., Krustev L. 1972. Enzyme - histochemical studies on rabbit - liver experimentally infected with Fasciola hepatica. Virchows Archiv. B. Cell Pathology, 11,334—342.
• Rule C. L, Behm C. A., Bygrave F. L. 1989. Aberrant energy-linked reactions in mitochondria isolated from the livers of rats infected with the liver fluke Fasciola hepatica. Biochemical Journal, 260, 517-523.
• Thorpe E. 1968. Histochemical study of the effect some anthelmintics in experimental fascioliasis in the rat. Annals of Tropical Medicine and Parasitology, 62, 361-371.
• Thorpe E., Ford E. J. H. 1969. Serum enzyme and hepatic changes in sheep infested with Fasciola hepatica. Journal of Pathology, 97,619-629.