EN
Tissue culture of leaf explants of Chrysanihemum cinerariaefolium Vis. was established on 6 solid media (RT and MS with various conbinations of hormones) to determine the most favourable medium content. Obtained callus culture were transferred onto regenerating media.Differentiation of tissue was the best on MS + kinetin 2 mg/dm3 + NAA 0,02 mg/dm3 + GA 10 mg/dm3. Regeneration of Ch.cinerariaefolium Vis. plant from shoot tips was achieved also. Presence of Pyrethrins in the obtained tissue culture was detected.