Developmental lead exposure impairs anxiolytic-like effect of diazapam and 8-OH-DPAT in male Wistar rats

• Autorzy: Nowak P , Kostrzewa R.M. , Adamus-Sitkiewicz B. , Brus R.
• Języki publikacji: EN

Abstrakty

EN

The effects of developmental lead (Pb²⁺) exposure on the anxiolytic-like effect of diazepam (5.0 mg/kg IP) and 8-OH-DPAT (0.3 mg/kg IP) were studied. Wistar dams were exposed to 250 ppm lead acetate in drinking water during pregnancy. Control rats were derived from dams that consumed tap water, and had no exposure to Pb²⁺ afterwards. Male offspring were tested at the age of 12 weeks. We studied the anxiolytic-like effect of diazepam and 8-OH-DPAT in an elevated plus maze device and the Vogel conflict test. Diazepam in doses of 5.0 mg/kg IP significantly increased the percentage of time spent on open arms in control rats being without effect in Pb²⁺-exposed animals. 8-OH-DPAT 0.3 mg/kg IP increased the percentage of time spent on open arms in both experimental groups (control and Pb²⁺), but the anxiolytic-like effect was much more pronounced in Pb²⁺-intoxicated animals. The benzodiazepine anxiolytic diazepam produced a significant effect in the Vogel conflict test in control rats. A 5.0 mg/kg dose of those drugs caused a significant increase in the number of electric shocks rats received. In the ontogenetically Pb²⁺-exposed rats diazepam also augmented the number of shocks accepted, but this effect was much less pronounced than in control animals. Conversely, 8-OH-DPAT at doses of 0.3 mg/kg IP was without effect in both tested groups as far as the anticonflict effect is concerned. The results of the present report demonstrated that exposure to Pb²⁺ during pregnancy induced hypersensitivity to 5-HT1A agonist mediated anxiolytic-like effect but attenuated that of benzodiazepine (diazepam).

Słowa kluczowe

EN

rat; male; Wistar rat; lead exposure; anxiety; diazepam; pregnancy; drinking water

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2008
• Tom: 17
• Numer: 5
• Opis fizyczny: p.751-756,fig.,ref.

Twórcy

autor

Nowak P

• Medical University of Silesia, Jordana 38, 41-808 Zabrze, Poland

autor

Kostrzewa R.M.

autor

Adamus-Sitkiewicz B.

autor

Brus R.

Bibliografia

• 1. DEVOTO P., FLORE G., IBBA A., FRATTA W., PANI L. Lead intoxication during intrauterine life and lactation but not during adulthood reduces nucleus accumbens dopamine release as studied by brain microdialysis. Toxicol. Lett. 121, 199, 2001.
• 2. MARCHETTI C. Molecular targets of lead in brain neurotoxicity. Neurotox. Res. 5, 221, 2003.
• 3. DEVI C.B., REDDY G.H., PRASANTHI R.P., CHETTY C.S., REDDY G.R. Developmental lead exposure alters mitochondrial monoamine oxidase and synaptosomal catecholamine levels in rat brain. Int. J. Dev. Neurosci. 23, 375, 2005.
• 4. BRUS R., SZKILNIK R., NOWAK P., KONECKI J., GŁOWACKA M., KASPERSKA A., OŚWIĘCIMSKA J., SAWCZUK K., SHANI J. Prenatal exposure of rats to lead, induce changes in the reactivity of the central dopaminergic, serotoninergic and muscarinic receptors, but not in glucose uptake in their offspring. Pharmacol. Rev. Comm. 9, 299, 1999.
• 5. BRUS R., SZKILNIK R., NOWAK P., OŚWIĘCIMSKA J., KASPERSKA A., SAWCZUK K., SŁOTA P., KWIECIŃSKI A., KUBAŃSKI N., SHANI J. Effect of lead and ethanol, consumed by pregnant rats, on behavior of their grown offsprings. Pharmacol. Rev. Comm. 10, 175, 1999.
• 6. SZKILNIK R., NOWAK P., WINIARSKA H., DURCZOK A., MALECKI S., RYCERSKA A., BRUS R., SHANI J. Effect of zinc on the reactivity of the central dopamine receptors in rats, prenatally exposed to lead. Pharmacol. Rev. Comm. 11, 319, 2001.
• 7. SZCZERBAK G., NOWAK P., KOSTRZEWA R.M., BRUS R. Maternal lead exposure produces long-term enhancement of dopaminergic reactivity in rat offspring. Neurochem. Res. 32, 1791, 2007.
• 8. DURCZOK A., SZKILNIK R., BRUS R., NOWAK P., LABUS L., KONECKI J., DRABEK K., KUBALLA G., RYCERSKI W., MENGEL K. Effect of organic mercury exposure during early stage of ontogenic development on the central dopaminergic system in adult rats. Pol. J. Environ. Stud. 11, 307, 2002.
• 9. KISZKA W., SZKILNIK R., BRUS R., NOWAK P., KONECKI J., DURCZOK A., MENGEL K., SHANI J. Prenatal exposure of rats to mercury induces changes in central dopaminergic activity and in glucose uptake by their offspring. Pharmacol. Rev. Comm. 12, 77, 2002.
• 10. NOWAK P., BRUS R., SZKILNIK R., LABUS L., WINIARSKA H., SHANI J. Exposure of pregnant rats to ethanol and cadmium modulates levels of biogenic amines in brains of their adult offspring. Pharmacol. Rev. Comm. 12, 229, 2002.
• 11. NOWAK P., DĄBROWSKA J., BORTEL A., BIEDKA I., KOSTRZEWA R.M., BRUS R. Prenatal cadmium and ethanol increase amphetamine-evoked dopamine release in rat striatum. Neurotoxicol. Teratol. 28, 563, 2006.
• 12. REDDY G.R., DEVI B.C., CHETTY C.S. Developmental lead neurotoxicity: alterations in brain cholinergic system. Neurotoxicology 28, 402, 2007.
• 13. VIRGOLINI M.B., CANCELA L.M., FULGINITI S. Behavioral responses to ethanol in rats perinatally exposed to low lead levels. Neurotoxicol. Teratol. 21, 551, 1999.
• 14. MOREIRA E.G., VASSILIEFF I., VASSILIEFF V.S. Developmental lead exposure: behavioral alterations in the short and long term. Neurotoxicol. Teratol. 23, 489, 2001.
• 15. JAAKO-MOVITS K., ZHARKOVSKY T., ROMANTCHIK O., JURGENSON M., MERISALU E., HEIDMETS L.T., ZHARKOVSKY A. Developmental lead exposure impairs contextual fear conditioning and reduces adult hippocampal neurogenesis in the rat brain. Int. J. Dev. Neurosci. 23, 627, 2005.
• 16. EISENBERG R., DAVIS S.L., ETTNER S., APPEL S., WILKEY S., VAN ROMPAY M., KESSLER R.C. Trends in alternative medicine use in the United States, 1990-1997: results of a follow-up national survey. J. Am. Med. Assoc. 280, 1569, 1998.
• 17. WHITING P.J. GABA-A receptors: a viable target for novel anxiolytics? Curr. Opin. Pharmacol. 6, 24, 2006.
• 18. GREEN A. Neuropharmacology of 5-hydroxytryptamine. Br. J Pharmacol. 147, 145, 2006.
• 19. NODA M., HIGASHIDA H., AOKI S., WADA K. Multiple signal transduction pathways mediated by 5-HT receptors. Mol. Neurobiol. 29, 31, 2004.
• 20. LANFUMEY L., HAMON M. 5-HT1 receptors. Curr. Drug Targets CNS Neurol. Disord. 3, 1, 2004.
• 21. BREMNER J.D., INNIS R.B., SOUTHWICK S.M., STAIB L., ZOGHBI S., CHARNEY D.S. Decreased benzodiazepine receptor binding in prefrontal cortex in combatrelated posttraumatic stress disorder. Am. J. Psychiatry 157, 1120, 2000.
• 22. NEUMEISTER A., YOUNG T., STASTNY J. Implications of genetic research on the role of the serotonin in depression: emphasis on the serotonin type 1A receptor and the serotonin transporter. Psychopharmacology (Berl). 174, 512, 2004.
• 23. PELLOW S., CHOPIN P., FILE S.E., BRILEY M. Validation of open:closed arm entries in an elevated plusmaze as a measure of anxiety in the rat. J. Neurosci. Methods. 14, 149, 1985.
• 24. VOGEL J.R., BEER B., CLODY D.E. A simple and reliable conflict procedure for testing anti-anxiety agents. Psychopharmacology, Berlin, 21, 1, 1971.
• 25. LECHIN F., VAN DER DIJS B., HERNÁNDEZ-ADRIÁN G. Dorsal raphe vs. median raphe serotonergic antagonism. Anatomical, physiological, behavioral, neuroendocrinological, neuropharmacological and clinical evidences: relevance for neuropharmacological therapy. Prog. Neuropsychopharmacol. Biol. Psychiatry 30, 565, 2006.
• 26. MILLAN M.J. The neurobiology and control of anxious states. Prog. Neurobiol. 70, 83, 2003.
• 27. WADA T., FUKUDA N. Effects of DN-2327, a new anxiolytic, diazepam and buspirone on exploratory activity of the rat in an elevated plus-maze. Psychopharmacology, Berlin, 104, 444, 1991.
• 28. PRZEGALIŃSKI E., TATARCZYŃSKA E., CHOJNACKA-WÓJCIK E. The influence of the benzodiazepine receptor antagonist flumazenil on the anxiolytic-like effects of CGP 37849 and ACPC in rats. Neuropharmacology 39, 1858, 2000.
• 29. NAITO H., NAKAMURA A., INOUE M., SUZUKI Y. Effect of anxiolytic drugs on air-puff-elicited ultrasonic vocalization in adult rats. Exp. Anim. 52, 409, 2003.
• 30. GRAEFF F.G., NETTO C.F., ZANGROSSI H. The elevated T-maze as an experimental model of anxiety. Neurosci. Biobehav. Rev. 23, 237, 1998.
• 31. XIAO C., GU Y., ZHOU C.Y., WANG L., ZHANG M.M., RUAN D.Y. Pb²⁺ impairs GABAergic synaptic transmission in rat hippocampal slices: a possible involvement of presynaptic calcium channels. Brain Res. 1088, 93, 2006.
• 32. BRAGA M.F., PEREIRA E.F., MARCHIORO M., ALBUQUERQUE E.X. Lead increases tetrodotoxin-insensitive spontaneous release of glutamate and GABA from hippocampal neurons. Brain Res. 826, 10, 1999.