Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry

• Autorzy: Stefanowicz P. , Boratynski J. , Kanska U. , Petry I. , Szewczuk Z.
• Języki publikacji: EN

Abstrakty

EN

Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation prod­ucts of proteins and peptides. We discuss influence of reaction conditions on the rate of glycation of lysozyme. We analyze for the first time collision induced dissociation spectra of the obtained peptide conjugates.

Słowa kluczowe

EN

high temperature; electrospray mass spectrometry; modified peptide; peptide; molecular mass; lysozyme; biomolecule; protein; glycation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 4
• Opis fizyczny: p.1137-1141,fig.

Twórcy

autor

Stefanowicz P.

• University of Wroclaw, F.Joliot-Curie 14, 50-383 Wroclaw, Poland

autor

Boratynski J.

autor

Kanska U.

autor

Petry I.

autor

Szewczuk Z.

Bibliografia

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• 6.Mole,D., Morgan, F., Bouhallab, S. & Leonil, J. (1998) Anal. Biochem. 259, 152-161.