Antioxidant activity of flavonoids of different polarity, assayed by modified ABTS cation radical decolorization and EPR technique

• Autorzy: Pawlak K , Bylka W. , Jazurek B. , Matlawska I. , Sikorska M. , Manikowski H. , Bialek-Bylka G.
• Języki publikacji: EN

Abstrakty

EN

Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the antioxidant activity of three flavonoids with different polarity: 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside) and I 3' II 8 biapigenine (amentoflavone), with nonpolar all-trans β-carotene used as standard carotenoid molecule. The ABTS [2,2'- azino-bis(3-ethylbenzthiazoline-6-sulphonic acid] cation radical decolorization assay was modified as follows: (1) measurements extended up to 8 days after preparation, (2) method adapted for flavonoids with different polarity and β-carotene, (3) concentrations in the 0.01-10 μM range of both trolox and antioxidants in order to use the same experimental conditions for both this technique and EPR measurement.

Słowa kluczowe

EN

free radical; antioxidant activity; flavonoids; polarity; ABTS method; EPR probe; radical scavenging activity; oxidation

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2010
• Tom: 52
• Numer: 1
• Opis fizyczny: p.97-104,fig.,ref.

Twórcy

autor

Pawlak K

• Poznan University of Technology, Nieszawska 13A, 60-695 Poznan, Poland

autor

Bylka W.

autor

Jazurek B.

autor

Matlawska I.

autor

Sikorska M.

autor

Manikowski H.

autor

Bialek-Bylka G.

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