Functionality versus strength - has functional selection taken place in the case of the ecdysteroid receptor response element?

• Autorzy: Grad I. , Kochman M. , Ozyhar A.
• Języki publikacji: EN

Abstrakty

EN

Nuclear receptors are ligand-dependent transcription factors responsible for con­trolling differentiation, growth and development of higher eukaryotes. Three amino acids within the recognition a-helix of the DNA-binding domain of the nuclear recep­tors constitute the so-called "P-box" which determines response element specificity. In the ultraspiracle (Usp) protein, which together with EcR forms the heterodimeric ecdysone receptor, the P-box residues are E19, G20 and G23. Substitution of E19, the most characteristic amino acid for estrogen receptor-like P-boxes, with alanine showed that the mutation did not appreciably alter the affinity of the wild-type Usp DNA-binding domain (UspDBDwT for a probe containing natural ecdysone response element (hsp27wt). Since in many cases E19 contacts a G/C base pair in position -4, which is absent in hsp27wt, we analysed the interaction of UspDBDwT, E19A and other P-box region mutants with the hsp27wt derivative which contains a G/C instead of an T/A base pair in position -4. UspDBDwT exhibited higher affinity for this ele­ment than for hsp27wt. Moreover, a different interaction pattern of P-box region mutants was also observed. Thus we conclude that the E19 residue of UspDBD is not involved in any hsp27wt sequence-discerning contacts. However, substitution of the hsp27wt T/A base pair in position –4 with G/C generates target sequence with distinct functional characteristics and possibly with a new specificity. These results could serve as a basis for understanding the role of the presence of a T/A or G/C base-pair in the position –4 in the two types of ecdysone response elements found in nature.

Słowa kluczowe

EN

protein-DNA interaction; ecdysteroid receptor; development; eukaryote; Drosophila; nuclear receptor; amino acid; growth; differentiation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 3
• Opis fizyczny: p.747-756,fig.

Twórcy

autor

Grad I.

• Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland

autor

Kochman M.

autor

Ozyhar A.

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