Wykrywanie paleczek Listeria monocytogenes i Salmonella sp. w rybach i produktach rybnych z uzyciem aparatu mini Vidas

• Autorzy: Zadernowska A , Laniewska-Trokenheim L. , Chajecka W.

Warianty tytułu

EN

Detection of Listeria monocytogenes and Salmonella sp. rods in fish and fish products using the mini Vidas system

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to evaluate the level of microbial contamination with Listeria monocytogenes and Salmonella sp. rods of fresh fish, smoked fish and sushi. Samples were purchased from retailers and restaurants. Indications were performed using a standard method, i.e. a horizontal method for detection and quantitative determination of Listeria monocytogenes according to PN-EN ISO 11290-1:1999 / A1: 2005 and Salmonella sp. according to the standard ISO-6579: 2003 and the mini Vidas immunoassay (bioMérieux) method. Mini-VIDAS is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for detection of pathogen antigens in food. It can be a rapid screening method alternative to time consuming classical ISO procedures based on isolation and identification. The mini-VIDAS-LMO detected 4 positive samples. The classical method confirmed these results for two samples. The mini-VIDAS-SLM detected 2 positive samples but they were excluded according to the classical method as a confirmation procedure. The study showed that the mini-Vidas can be used for such studies; however, it is necessary to confirm the results of the standard method, because it can give false positive results.

Słowa kluczowe

PL

zywnosc; ryby; produkty rybne; ryby surowe; ryby wedzone; sushi; zanieczyszczenia zywnosci; zanieczyszczenia bakteryjne; Salmonella; Listeria monocytogenes; wykrywanie; metody immunoenzymatyczne; aparat mini Vidas; metoda klasyczna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 04
• Opis fizyczny: s.264-267,tab.,bibliogr.

Twórcy

autor

Zadernowska A

• Uniwersytet Warminsko-Mazurski w Olsztynie, pl.Cieszynski 1, 10-726 Olsztyn

autor

Laniewska-Trokenheim L.

autor

Chajecka W.

Bibliografia

• 1. Ben Embarek P. K.: Presence, detection and growth of Listeria monocytogenes in seafoods: A review. Int. J. Food Microbiol. 1994, 23, 17-34.
• 2. Chatopadhyay P.: Fish - catching and handling. Robinson R. K.: Encyclopedia of Food Microbiology. Academic Press, London 2000, 2, 1547.
• 3. Elizaquivel P., Gabaldon J. A., Nazar R.: Comparative evaluation of RTi-PCR and Mini-VIDAS SLM system as predictive tools for the routine detection of Salmonella spp. in naturally contaminated food products. Food Anal. Methods 2009, 2, 102-109.
• 4. Feder I., Nietfeld J. C., Galland J., Yeary T., Sargeant J. M., Oberst R., Tamplin M. L., Luchansky J. B.: Comparison of cultivation and PCR-hybridization for detection of Salmonella in porcine fecal and water samples. J. Clin. Microbiol. 2001, 39, 2477-2484.
• 5. Ferreti R., Mannazzu I., Cocolin L., Comi G., Clementi F.: Twelve-hour PCR-based method for detection of Salmonella spp. in food. Appl. Environ. Microbiol. 2001, 67, 977-978.
• 6. González-Rodríguez M., Sanz J., Santos J., Otero A., García-López M.: Numbers and types of microorganisms in vacuum-packed cold-smoked freshwather fish and the retail level. Int. J. Food Microbiol. 2002, 77, 161-168.
• 7. Heintz M. L., Johnson J. M.: The incidence of Listeria spp., Salmonella spp., and Clostridium botulinum in smoked fish and shellfish. J. Food Prot. 1998, 61, 318-323.
• 8. Hoffman A. D., Gall K. L., Norton D. M., Wiedmann M.: Listeria monocytogenes contamination patterns for the smoked fish processing environment and for raw fish. J. Food Prot. 2003, 66, 52-60.
• 9. Jemmi T., Keusch A.: Occurrence of Listeria monocytogenes in freshwater fish farms and fish-smoking plants. Food Microbiol. 1994, 11, 309-316.
• 10. Jin M., Kusunoki K., Ikejima N., Arai T., Irikura Y., Suzuki K., Hirata I., Kokubo Y., Maruyama T.: Incidence of Listeria monocytogenes in smoked salmon. Jpn. J. Food Microbiol. 1994, 11, 107-111.
• 11. Johansson T., Rantala L., Palmu L., Honkanen-Buzalski T.: Occurrence and typing of Listeria monocytogenes strains in retail vacuum-packed fish products and production plant. Int. J. Food Microbiol. 1999, 47, 111-119.
• 12. Jørgensen L. V., Huss H. H.: Prevalence and growth of Listeria monocytogenes in naturally contaminated seafood. Int. J. Food Mikrobiol. 1998, 42, 127-131.
• 13. Metz H.: Water as a vector of infection: waterborne bacteria. Zentralbl. Bakteriol. Mikrobiol. Hyg. B. 1980, 172, 255-274.
• 14. Miettinen M. K., Palmu L., Björkroth K. J., Korkeala H.: Prevalence of Listeria monocytogenes in broilers at the abattoir, processing plant and retail level. J. Food Prot. 2001, 64, 994-999.
• 15. Nowotny L., Dvorska L., Lorencova A., Beran V., Pavlik I.: Fish a potencial source of bacterial pathogenes for human beings. Vet. Med. - Czech. 2004, 49, 343-358.
• 16. Osek J.: Listeria monocytogenes - groźny czynnik zaka¿eñ pokarmowych. Medycyna Wet. 2005, 61, 243-248.
• 17. Pilet M. F., Dousset X., Barre´ R., Novel G., Desmazeaud M., Piard J. C.: Evidence for two bacteriocins produced by Carnobacterium piscicola and Carnobacterium divergens isolated from fish and active against Listeria monocytogenes. J. Food Prot. 1994, 58, 256-262.
• 18. Rorvick L. M.: Listeria monocytogenes in the smoked salmon industry. Int. J. Food Microbiol. 2000, 62, 183-190.
• 19. Seremak-Bulge J.: Rynek i spożycie ryb w 2007 roku oraz perspektywy ich rozwoju. Przegląd rybacki. 2007, 10, 16-19.
• 20. Sikorski Z. E.: Listeria monocytogenes w rybach wêdzonych. Medycyna Wet. 1996, 52, 295-297.
• 21. Sikorski Z. E.: The nutritional and safety aspects of smoked fish. Bull. Sea Fish. Inst. 1998, 1, 137.
• 22. Sun E., Aristimun C., Fernandez-Galian B.: Activity of smoke wood condensates against Aeromonas hydrophila and Listeria monocytogenes in vacuum-packaged, cold-smoked rainbow trout stored at 4°C. Food Res. Int. 2003, 36, 111-116.
• 23. Toze S.: Reuse of effluent water - benefits and risks. Agric. Water Manage 2006, 80, 147-159.
• 24. Vishwanath W., Lillabati H., Bijen M.: Biochemical, nutritional and microbiological quality of fresh and smoked mudeel fish Monopterus albus: a comparative study. Food Chem. 1998, 61, 153-156.