Izolacja shiga-toksycznych szczepow Escherichia coli od zdrowego bydla z regionu Dolnego Slaska

• Autorzy: Sobieszczanska B. , Gryko R. , Dobrowolska M. , Blaszkowska M. , Twardon J.

Warianty tytułu

EN

Isolation of shiga toxin-producing Escherichia coli strains from healthy cattle of the region of Lower Silesia

• Języki publikacji: PL

Abstrakty

PL

Podstawowym rezerwuarem szczepowi', coli wytwarzających toksyny shiga jest bydło. W województwie dolnośląskim wśród zdгоwego bydła wykazano nosicielstwo pałeczek E. coli wytwarzających toksyny shiga, które mogą stanowić źródło zakażeń ludzi.

EN

The aim of the study was to determine if cattle from the region of Lower Silesia is the reservoir of shiga toxin-producing E. coli strains (STEC) and the analysis of virulence factors of isolated STEC strains. The ability of tested animal strains to shiga toxin synthesis was analysed in cytotoxicity assay in vitro on Vero cell line and then confirmed by detection of shiga toxin-encoding genes by PCR. STEC strains were isolated from 12 (15,2%) of animals examined, 21,4% of these strains were obtained from 9 of 42 calves, and 8,1% from 3 of 37 cows. Most of STEC isolated (75%) was enterohemolysin- producing. The cattle from the region of Lower Silesia is the reservoir of pathogenic for humans sorbitol-fermenting non-0157 STEC strains.

Słowa kluczowe

PL

woj.dolnoslaskie; bydlo; zwierzeta zdrowe; Escherichia coli; toksyny shiga; zakazenia; zrodla zakazenia; czlowiek; zagrozenia zdrowia

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 4
• Opis fizyczny: s.369-375,rys.,tab.,bibliogr.

Twórcy

autor

Sobieszczanska B.

• Akademia Medyczna we Wroclawiu, ul.Chalubinskiego 4, 50-368 Wroclaw

autor

Gryko R.

autor

Dobrowolska M.

autor

Blaszkowska M.

autor

Twardon J.

Bibliografia

• 1. Amnion A, Peterson LR, Karch H. A large outbreak of hemolytic uremic syndrome caused by an unusual sorbitol-fermenting strain E. coli 0157:H-. J Infect Dis 1999; 179: 1274-7.
• 2. Bertin Y, Boukhors K, Pradel N i inni. Stx2 subtyping of shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new stx2 subtype and correlation with additional virulence factors. J Clin Microbiol 2001; 39: 3060-5.
• 3. Beutin L, Montenegro M, Řrskov l i inni. Close association of verotoxin (shiga-like toxin) production with enterohaemolysin production in strains of Escherichia coli. J Clin Microbiol 1989; 27: 2559-64.
• 4. Bielaszewska M, Schmidt H, Karmali MA i inni. Isolation and characterization of sorbitol-fermenting shiga toxin-producing Escherichia coli 0157:H- strains in the Czech Republik. J Clin Microbiol 1998; 36: 2135-7.
• 5. Blanco M, Blanco JE, Blanco J i inni. Prevalence and characteristics of Escherichia coli serotype 0157.H7 and other verotoxin-producing E. coli in healthy cattle. Epidemiol Infect 1996; 117: 251-7.
• 6. Griffin PM, Tatixe RV. The epidemiology of infections caused by Escherichia coli 0157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev 1991; 13: 60-98.
• 7. Heuvelink AE, van den Biggelaar FL, Zwartkruis-Nahuis JT i inni. Occurrence of verocytotoxin- producing Escherichia coli 0157 on Dutch diary farms. J Clin Microbiol 1998; 36: 3480-7.
• 8. James CE, Stanley KN, Allison HE i inni. Lytic and lysogenic infection of diverse Escherichia coli and Shigella strains with a verocytotoxingenic bacteriophage. Appl Environ Microbiol 2001; 67: 4335-7.
• 9. Konowalchuk J, Speirs JT, Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun 1977; 18: 775-9.
• 10. Le Jeune JT, Besser TE, Hancoc DD. Cattle water troughs as reservoirs of Escherichia coli 0157. Appl Environ Microbiol 2001; 67: 3053-7.
• 11. Linqvist R. Preparation of PCR samples from food by a rapid and simple centrifugation technique evaluated by detection of Escherichia coli 0157:117. Int J Food Microbiol 1997; 37: 73-8.
• 12. Mainil J. Shiga/verocytotoxins and shiga/verotoxigenic Escherichia coli in animals. Vet Res 1999; 30: 235-57.
• 13. Muniesa M, Jofre J. Abundance in sewage of bacteriophages that infect Escherichia coli 0157.H7 and that carry the shiga toxin 2 gene. Appl Environ Microbiol 1998; 64: 2443-8.
• 14. Muniesa M, Lucena F, Jofre A. Comparative survival of free shiga toxin 2 - encoding phages and Escherichia coli strains outside the gut. Appl Environ Microbiol 1999; 65: 5615-8.
• 15. Osek J. Molecular characterization of Shiga toxin-producing Escherichia coli 0157 strains isolated in Poland. Res Vet Sci 2001; 70: 175-7.
• 16. Osek J. Identification of eae genes in Escherichia coli strains isolated from pigs with postweaning diarrhea. Vet Ree 2001; 148: 241-3.
• 17. Osek J. Identification of Escherichia coli O157:117 - strains from pigs with postweaning diarrhea and amplification of their virulence marker genes by PCR. Vet Ree 2002; 150: 689-92.
• 18. Phillips AD, Navabpour S, Hicks S i inni. Enterohemorrhagic Escherichia coli 0157.H7 target Peyer's patches in humans and cause attaching/effacing lesions in both, human and bovine intestine. Gut 2000;47:377-81.
• 19. Pradel N, Livrell V, De Champs C i inni. Prevalence and characterization of shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one year prospective study in France. J Clin Microbiol 2000; 38: 1023-31.
• 20. Sobieszczańska В, Gryko R, Kuzko K i inni. Prevalence of non-0757 Escherichia coli strains among shiga-like toxin-producing (SLTEC) isolates in the region of Lower Silesia, Poland. Scand J Infect Dis 2004; 36:219-21.
• 21. Wagner PL, Acheson DW, Waldor MK. Isogenic lysogens of diverse shiga toxin 2 - encoding bacteriophages produce markedly different amounts of shiga toxin. Infect Immun 1999; 67: 6710-4.
• 22. Weaver JW, Rowe MT. Effect of non-target cells on the sensitivity of the PCR for Escherichia coli 0157-.H7. Lett Appl Microbiol 1997; 25: 109-12.
• 23. Wieler LH, Busse В, Steinrück H i inni. Enterohemorrhagic Escherichia coli (EHEC) strains of serogroup O118 display three distinctive clonal groups of EHEC pathogens. J Clin Microbiol 2000; 38:2162-9.
• 24. Zhang X, McDaniel AD, Wolf LG i inni. Quinolone antibiotics induce shiga toxin-encoding bacteriophages, toxin production, and death in mice. J Infect Dis 2000; 181: 664-70.