Epidermal growth factor [EGF] expression in human salivary glands. An immunohistochemical study

• Autorzy: Dubiel B , Mytar B. , Tarnawski A. , Zembala M. , Stachura J.
• Języki publikacji: EN

Abstrakty

EN

Epidermal growth factor (EGF) is a biologically active peptide involved in differentiation, growth, regeneration and repair of human and animal tissues. Quantitative biochemical studies showed in man the highest concentration of EGF in the parotid gland. The aim of the present study was to define EGF immunolocalization in the individual segments of the human major salivary glands (salivon). The material consisted of sections obtained from the surgically removed salivary glands: parotid, submaxillary and sublingual. Immunohistochemical studies were performed by PAP method using monoclonal antibody against human epidermal growth factor. EGF expression was found almost exclusively in the efferent pathways of the salivary glands, mostly in the intercalated ducts and Pflüger salivary tubules. These segments of the salivon are most developed in the parotid gland in which the staining was stronger than in other salivary glands.

Słowa kluczowe

EN

epidermal growth factor; salivon; immunolocalization; salivary gland; immunohistochemistry; growth

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1992
• Tom: 43
• Numer: 1
• Opis fizyczny: p.21-32,fig.

Twórcy

autor

Dubiel B

• University Medical School, Krakow, Poland

autor

Mytar B.

autor

Tarnawski A.

autor

Zembala M.

autor

Stachura J.

Bibliografia

• 1. Fisher DA, Lakshamanan J. Metabolism and effects of epidermal growth factor and related growth factors in mammals. Endocrine Review 1990; 11: 418-442.
• 2. Rao RK. Biologically active peptides in the gastrointestinal lumen. Minireview. life Sciences 1991; 48: 1685-1704.
• 3. Beerstecher HJ, Huiskens-van der Meij C, Warnaar So. An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor. J. Histochem. Cytochem. 1988; 36: 1153-1160.
• 4. Konturek SJ, Dembiński A, Warzecha Z, Brzozowski T, Gregory H. Role of epidermal growth factor in healing of chronic gastroduodenal ulcers in rats. Gastroenterology 1988; 94: 1300-1307.
• 5. Olsen PS, Pulsen SS, Kirkegaard P, Nexo E. Role of submandibular saliva and epidermal growth factor in gastric cytoprotection. Gastroenterology 1984; 87: 103-108.
• 6. Seifert G, Caselitz J. Epithelial salivary gland tumors: tumor markers. In: Progress in Surgical Pathology. Ed. CM. Fenoglio-Preiser, M. Wolff, F. Rilke. Springer Verlag Berlin, Heidelberg, New York, Tokyo 1989.
• 7. Kohler G. Milstein C. Continuous culture of fused cells secreting antibody of predefined specifity. Nature 1975; 265: 497.
• 8. Kingsnorth AN, Volwes R. Epidermal growth factor augments intestinal wound healing in pigs. Gastroenterology 1989: 96: A 257.
• 9. Konturek SJ. Role of growth factors in gastroduodenal protection and healing of peptic ulcers. Gastroenterol. Clin. N. Am. 1990; 19: 41-65.
• 10. Konturek SJ, Brzozowski T, Konturek PK, Majka J, Dembiński A. Role of salivary glands and epidermal growth factor (EGF) in gastric secretion and mucosal integrity in rats exposed to stress. Regulatory Peptides 1991; 32: 203-215.
• 11. Ostrowski K. Histologia. PZWL Warszawa 1988; pp. 464-469.
• 12. Seifert G, Miehlke A, Haubrich J, Chilla R. Diseases of the Salivary Glands. Ed. Georg Thieme Verlag-Stuttgart, New York. Thieme Inc.-New York 1986; 7-13.
• 13. Thesleff I, Viinikka L, Saxen L, Lehtonen E, Perheentupa J. The parotid gland is the main source of human salivary epidermal growth factor. life Sciences 1988; 43: 13-18.
• 14. Wright NA, Poulsom R, Stamp G. et al. Epidermal growth factor (EGF/URO) induces expression of regulatory peptides in damaged human gastrointestinal tissues. Rapid communication. J. Pathol. 1990; 162: 279-284.