Tetracyclines and their epimers in animal tissues by high-performance liquid chromatography

• Autorzy: Gajda A , Posyniak A.
• Języki publikacji: EN

Abstrakty

EN

An analytical method for the simultaneous determination of tetracycline (TC), oxtetracycline (OTC), chlortetracycline (CTC) with their 4-epimers, and doxycycline (DC) in animal tissues has been developed and validated. The extraction of the analytes from biological matrice was carried out with a 0.02 M oxalic acid (pH=4.0). The samples were cleaned up by using a solid phase extraction procedure with polymeric cartridges. Chromatographic separation was achieved on a C 18 analytical column using a mobile phase consisting of acetonitrile, methanol and 0.02 M oxalic acid in gradient mode. Detection was carried out by UV detector at λ = 355 nm. The method has been validated according to the Commission Decision 2002/657/EC. The recoveries of the analytes from the spiked samples were 50%-80%. The decision limits (CCα) were from 110 to 125 µg/kg and the detection capabilities (CCß) were from 120 to 155 µg/kg, depending on the analytes. The prepared method was successfully verified in the National Residue Control Programme.

Słowa kluczowe

EN

animal tissue; tetracycline; oxtetracycline; chlortetracycline; doxycycline; 4-epimer; determination; high performance liquid chromatography; validation; quantitative determination; veterinary medicine; Directive 2002-657-EC; muscle sample

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 2
• Opis fizyczny: p.263-267,fig.,ref.

Twórcy

autor

Gajda A

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Posyniak A.

Bibliografia

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