EN
Tissue cultures of Tagetes erecta Linn. were initiated from leaves and flowers on MS and RT media with various cytokinins and auxins combinations Cultures were maintaned at 25°C, light intensity 3000 lux and photo-period 16 h. Obtained callus tissue was transferred on regeneration medium (MS plus various combinations of growth regulators) for differentiation. The best organogenesis was observed on MS plus BA 5 mg/dm3 plus IAA 3mg/dm3 medium. Rooted plantlets were transferred to sterilized soil. Regeneration of plant from leaves tissue culture was not successful. The presence of pyrethrins in callus was detected.