Simple and rapid technique to detect PVY presence in some Solanaceae plants

• Autorzy: Krzymowska M , Hennig J.
• Języki publikacji: EN

Abstrakty

EN

The study of plant-virus interactions requires a method which enables not only the detection of virus presence, but also its distribution in the infected plant tissue. We adopted a tissue print immunoblot technique for localization of potato virus Y (PVY) coat protein (CP) in infected potato and tobacco tissue. In potato stem cross-sections PVY CP was detected on the whole surface of the prints, however significantly higher concentration was observed in epidermis and phloem tissue. In the petiole of infected tobacco leaves the presence of viral protein was confined mainly to peripheral layers of parenchyma and epidermis. In phloem tissue the signal was also visible but it was significantly weaker. In our hands this approach is highly specific and gives resolution on the level of individual cells, thus it can be applied in several fields. As potyviral capsid proteins are involved in several events of pathogenesis the technique for immunolocalization of PVY CP could provide information which will shed new light on the virology of PVY.

Słowa kluczowe

EN

infection; virus localization; Solanaceae; plant tissue; potato virus Y; plant-virus interaction; plant

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 2
• Opis fizyczny: p.95-99,fig.

Twórcy

autor

Krzymowska M

• Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Hennig J.

Bibliografia

• Cassab G.I. and Varner J.E. 1987. Immunocytolocalization of extension in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper. J. Cell Biol., 105: 2581–2588.
• Cronin S., Verchot J., Haldeman-Cahil R., Schaad M.C. and Carrington J.C. 1995. Long-distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell, 7: 549–559.
• De Bokx J.A. and Huttinga H. 1981. Potato virus Y. No. 242. In: Descriptions of Plant Viruses. Commonwealth Mycological Institute and Association of Applied Biologists. Kew, England.
• Hari V. 1995. The potyviridae. In: R.P. Singh, U.M. Singh and K. Kohmoto (Eds.), Pathogenesis and host specificity in plant diseases. Histopathological, biochemical and molecular bases. Volume III: Viruses and viroids. Pergamon: 1–18.
• Klessig D.F. and Malamy J. 1994. The salicylic acid signal in plants. Plant Mol. Biol., 26: 1439–1458.
• Leisner S.C. and Howell S.H. 1993. Long-distance movement of viruses in plants. Trends Microbiol., 1: 314–317.
• Malnoë P., Farinelli L., Collet G.F. and Reust W. 1994. Small — scale field tests with transgenic potato, cv. Bintje, to test resistance to primary and secondary infections with potato virus Y. Plant Mol. Biol., 25: 963–975.
• Matthews R.E.F. 1991. Plant Virology. Academic Press, San Diego, CA.
• McClure B.A. and Guilfoyle T. 1989. Tissue print hybridization. A simple technique for detecting organ-and tissue-specific gene expression. Plant Mol. Biol., 12: 517–524.
• Schopfer P. 1994. Histochemical demonstration and localization of H₂O₂ in organs of higher plants by tissue printing on nitrocellulose paper. Plant Physiol., 104: 1269–1275.