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2006 | 58 | 1 |

Tytuł artykułu

Paleczki Gram-ujemne wytwarzajace metalo-beta-laktamazy izolowane od pacjentow szpitalnych i pozaszpitalnych, wykryte przy zastosowaniu metody Etest MBL

Warianty tytułu

EN
Metallo-beta-lactamase-producing Gram-negative rods isolated from inpatients and outpatients, detected using Etest MBL

Języki publikacji

PL

Abstrakty

PL
W latach 2001-2005 z próbek materiału klinicznego pobranych od pacjentów szpitalnych i ambulatoryjnych wyizolowano 104 szczepy Gram-ujemnych pałeczek opornych na karbapenemy (imipenem i meropenem). Zastosowano fenotypową metodę Etest® MBL do wykrywania szczepów wytwarzających metalo-beta-laktamazy (MBL). Wśród badanych izolatów było 36 MBL-do- datnich stanowiących 34,6% ogółu szczepów opornych na antybiotyki kar- bapenemowe.
EN
The aim of presented study was to detect MBL-positive strains in a group of clinical carbapenem- resistant strains isolated from inpatients and outpatients during last four years. From the beginning of November 2001 to the end of October 2005, one hundred and four strains resistant to carbapenem antibiotics - imipenem and meropenem were cultured from clinical samples obtained from patients of the Infant Jesus Clinical Hospital Centre for Trauma Treatment in Warsaw and from patients of outpatient clinics. Strains were identified and their susceptibility to antibacterial agents was determined in the automatic ATB Expression system (bioMérieux®). Resistance to imipenem and meropenem was confirmed with a disc diffusion method. Production of metallo-beta-lactama- ses (MBL) was examined with the use of Etest® MBL (AB Biodisk, Sweden), and extended-spectrum beta-lactamases (ESBL) by means of following procedures: DDST and / or DD (four variants) (Oxoid Ltd., England). MBL-positive strains (36) were cultured in cases of infections in adult patients (35 strains) and in a child (1 strain). Majority of strains belonged to the species P. aeruginosa (27), several strains - to the species P. putida (6) and remaining strains - to P. stutzeri, A. xylosoxidans, and E. cloacae ( 1 strain of each species). Four strains were producers of MBL-type and ESBL-type beta-lactamases. According to our knowledge and accessible literature described strains (except one paediatric strain) are the first MBL-positive strains isolated from adult hospitalized patients and adult ambulatory patients in Poland. Additionally, MBL-positive E. cloacae strain is probably the first MBL producer isolated in Poland, which belongs to the group of enteric rods. MBL-producing strains of Gram-negative rods, detected by phenotypic Etest® MBL method, will be verified with genetic procedures.

Wydawca

-

Rocznik

Tom

58

Numer

1

Opis fizyczny

s.73-81,tab.,bibliogr.

Twórcy

autor
  • Akademia Medyczna w Warszawie, ul.T.Chalubinskiego 5, 02-004 Warszawa
autor

Bibliografia

  • 1. Ambler RP. The structure of beta-lactamases. Philos Trans R Soc Lond В Biol Sci 1980; 18: 321-31.
  • 2. Arakawa Y, Shibata N, Shibayama K i inni. Convenient test for screening metallo-ß-lactamase- producing gram-negative bacteria by using thiol compounds. J Clin Microbiol 2000; 38: 40-3.
  • 3. Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 1995; 39: 1211-33.
  • 4. ETM, Etest Technical Manual, AB Biodisk, Solna, Sweden. Edition 2001.
  • 5. Fritsche TR, Sader HS, Toleman MA i inni. Emerging metallo-ß-lactamase-mediated resistances: a summary report from the worldwide SENTRY Antimicrobial Surveillance Program. Clin Infect ' Dis 2005; 41(Suppl 4): S276-8.
  • 6. Helfand MS, Bonomo RA. Current challenges in antimicrobial chemotherapy: the impact of extended-spectrum ß-lactamases and metallo-ß-lactamases on the treatment of resistant Gram-negative pathogens. Curr Opin Pharmacol 2005; 5: 1-7.
  • 7. Hryniewicz W, Sulikowska A, Szczypa K i inni. Rekomendacje doboru testów do oznaczania wrażliwości bakterii na antybiotyki i chemioterapeutyki. Post Mikrobiol 2005; 44: 175-92.
  • 8. Jones RN, Biedenbach DJ, Sader HS i inni. Emerging epidemie of metallo-ß-lactamase-mediated resistances. Diagn Microbiol Infect Dis 2005; 51: 77-84.
  • 9. Laudy AE. Karbapenemazy - enzymy mogące hydrolizować szerokie spektrum ß-laktamow. Zakażenia 2003; 3(8): 32-8.
  • 10. Lee K, Chong Y, Shin HB i inni. Modified Hodge and EDTA-disk synergy tests to screen metallo- ß-lactamase-producing strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect 2000; 7: 88-102.
  • 11. National Committee for Clinical Laboratory Standards (NCCLS). Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically, 6th ed. Approved standard Document M7-A6. Wayne, PA 2003.
  • 12. Nordmann P, Poirel L. Emerging carbapenemases in Gram-negative aerobes. Clin Microbiol Infect Dis 2002; 8: 321-31.
  • 13. Patzer J, Toleman MA, Deshpande LM i inni. Pseudomonas aeruginosa strains harbouring an unusual blaVIM-4 gene cassette isolated from hospitalized children in Poland (1998-2001). J Antimicrob Chemother 2004; 53: 451-6.
  • 14. Patzer JA, Toleman MA, Grzesik A i inni. The diverse integron structures disseminating VIM genes in Poland. Clin Microbiol Infect 2005; ll(Suppl 2): 100.
  • 15. Patzer J, Toleman M, Kaminska D i inni. VIM-1 or VIM-1 + VIM-2 metallo-lactamase-producing Pseudomonas aeruginosa isolated from hospitalized children in Poland (1997-2002). Clin Microbiol Infect 2003; 9(Suppl 1): 245.
  • 16. Rokosz A, Sawicka-Grzelak A, Łuczak M. Zastosowanie czterech wariantów testu krążka diagnostycznego (CD01, CD02, CD03, CD04) do wykrywania ESBL-dodatnich szczepów Gram-ujemnych pałeczek. Med Dośw Mikrobiol 2005; 57: 77-83.
  • 17. Walsh TR. The emergence and implications of metallo-beta-lactamases in Gram-negative bacteria. Clin Microbiol Infect 2005; ll(Suppl 6): 2-9.
  • 18. Walsh TR, Bolmstrom A, Qwarnstrom A, Gales A. Evaluation of a new Etest for detecting metallo- ß-lactamases in routine clinical testing. J Clin Microbiol 2002; 40: 2755-9.
  • 19. Walsh TR, Toleman MA, Hryniewicz W i inni. Evolution of an integron carrying bla VIM-2 in Eastern Europe: report from the SENTRY Antimicrobial Surveillance Program. J Antimicrob Chemother 2003; 52: 116-9.
  • 20. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-b-lactamases: the quiet before the storm? Clin Microbiol Rev 2005; 18: 306-25.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-1ae34ee0-39ea-4bc6-b8aa-69571a504a57
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