A novel isoform of human lymphoid enhancer-binding factor-1 [LEF-1] gene transcript encodes a protein devoid of HMG domain and nuclear localization signal

• Autorzy: Kobielak A. , Kobielak K. , Trzeciak W.H.
• Języki publikacji: EN

Abstrakty

EN

Lymphoid enhancer-binding factor-1 (LEF-1), a member of the high mobility group (HMG) family of proteins, regulates expression of T-cell receptor-a gene and is one of the key regulatory molecules in the epithelial-mesenchymal interactions during embryonic de­velopment. Among others, LEF-1 regulates expression of cytokeratin genes involved in formation of hair follicles and the gene encoding the cell-adhesion molecule E-cadherin. Transcription factor LEF-1, which acts as a dimer, binds ß-catenin and is involved in signal transduction by the wnt pathway. We have cloned and sequenced a novel isoform of human LEF-1 gene transcript. This isoform encodes a truncated protein devoid of HMG domain and nuclear localization sig­nal but retaining ß-catenin binding domain. This isoform might either act in a domi­nant-negative manner by interfering with native LEF-1, or might bind ß-catenin in the cytosol, which would result in attenuation of the signals transmitted by theLEF-ß-catenin pathway.

Słowa kluczowe

EN

cell adhesion molecule; regulatory molecule; cytokeratin gene; man; embryonic development; gene encoding; protein; expression; lyphoid enhancer binding factor-1; epithelial-mesenchymal interaction; T cell

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.221-226,fig.

Twórcy

autor

Kobielak A.

• University of Medical Sciences, H.Swiecickiego 6, 60-781 Poznan, Poland

autor

Kobielak K.

autor

Trzeciak W.H.

Bibliografia

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