Thiamine prevents X-ray induction of genetic changes in human lymphocytes in vitro

• Autorzy: Konopacka M. , Rogolinski J.
• Języki publikacji: EN

Abstrakty

EN

The effects of thiamine (vitamin B1) on the level of spontaneous or radia­tion-induced genetic changes in human lymphocytes in vitro were studied. Cul­tured lymphocytes were exposed to increasing concentrations of thiamine (0-500 Ug/ml) and irradiated with X-rays. The DNA damage was estimated as the frequency of micronuclei and apoptotic or necrotic morphological changes in fixed cells. The results show that thiamine alone did not induce genetic changes. A significant decrease in the fraction of apoptotic and necrotic cells was observed in lymphocytes irradiated in the presence of vitamin B1 at concentrations between 1-100 ug/ml compared to those irradiated in the absence of thiamine. Vitamin B1 at 1 and 10 Ug/ml decreased also the extent of radiation-induced formation of micronuclei. Vita­min B1 had no effect on radiation-induced cytotoxicity as measured by nuclear divi­sion index. The results indicate that vitamin B1 protects human cells from radia­tion-induced genetic changes.

Słowa kluczowe

EN

man; X-radiation; lymphocyte; oxidative stress; genetic change; necrosis; apoptosis; micronucleus; thiamine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.839-843,fig.,ref.

Twórcy

autor

Konopacka M.

• Institute of Oncology, Armii Krajowej 15, 44-101 Gliwice, Poland

autor

Rogolinski J.

Bibliografia

• Ames BN, Shigenaga MK, Hagen TM. (1993) Proc Natl Acad Sci USA.; 90: 7915-22. Beckman KB, Ames BN. (1998) Physiol Rev.; 78: 547-81. Collins AR. (1999) Bioessays.; 21: 238-56. Draper HH. (1998) J Nutr.; 118: 1420-1.
• Dusinska M, Kazimirowa A, Barancokova M, Beno M, Smolkowa B, Horska A, Raslova K, Wsolova L, Collins AR. (2003) Mutagenesis.; 18: 371-7.
• Fenech M, Morley AA. (1985) Mutat Res.; 147: 29-36.
• Fenech M, Crott J, Turner J, Brown S. (1999) Mutagenesis.; 14: 605-12.
• Heinrich E, Getoff N. (2002) Anticancer Res.; 22: 927-30.
• Hu ML, Chen TK, Lin YF. (1995) Chem-Biol Interact.; 97: 63-73.
• Jaruga P, Jaruga B, Gackowski D, Olczak A, Halota W, Pawlowska M, Olinski R. (2002) Free Radic Biol Med.; 32: 414-20.
• Konopacka M, Rzeszowska-Wolny J. (2001) Mutat Res. ; 491: 1-7. Konopacka M, Widel M, Rzeszowska-Wolny J. (1998) Mutat Res. ; 417: 85-94.
• Konopacka M, Palyvoda O, Rzeszowska-Wolny J. (2002) Teratogenesis CarcinogMutagen.; 22: 443-50. Sarma L, Kesavan PC. (1993) Int JRadiatBiol.; 63: 759-64. Singh NP. (1997) Mutat Res.; 375: 195-203 .