Detection of Trichinella spiralis antigens in urine of men and animals

• Autorzy: Machnicka B , Prokopowicz D. , Dziemian E. , Kolodziej-Sobocinska M.
• Języki publikacji: EN

Abstrakty

EN

The practical inability to diagnose Trichinella spiralis antibodies in man before day 20 post infection (dpi) has stimulated interest in the development of immunodiagnostic test to detect circulating antigens. Our previous experience showed that soon after infection immune complexes as well as uncomplexed parasite antigens in sera of infected rats could be detected. To diagnose the presence of antigen in urine, double sandwich-capture ELISA was applied using a peroxidase-conjugated rabbit immunoglobulin to T. spiralis larval antigens. The plates were coated with metabolic (AES) or somatic (AS) larval antigens. Mice were infected with 500 T. spiralis larvae. The urine samples from experimentally infected mice taken from 1 to 41 dpi. and the urine samples from patients of the Clinical Hospital in Białystok taken from 3 to 120 dpi were examined. Before testing, the urine samples were heated for 6 min. at 100°C and centrifuged for 6 min. at 5000 g, supematants were used in ELISA. The presence of T. spiralis antigens in mice urine samples was detected between 6-26 days post infection (dpi) using double sandwich-capture ELISA. All samples taken later were negative as samples taken from uninfected mice. 3 from 9 human urine samples taken 3-10 dpi were positive, the remaining samples taken 3-10 and 10-30 dpi showed values near to "cut-off". In both mice and human urine samples the higher level of antigens was detected in ELISA when somatic larval antigen was used. The T. spiralis antigens were present in urine of infected men and mice in the first phase of infection.

Słowa kluczowe

EN

parasite; antigen; man; immunodiagnostic test; animal; urine; Trichinella spiralis; detection; antibody

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 2
• Opis fizyczny: p.217-225,fig.,ref.

Twórcy

autor

Machnicka B

• Polish Academy of Sciences, Twarda str 51-55, 00-818 Warsaw, Poland

autor

Prokopowicz D.

autor

Dziemian E.

autor

Kolodziej-Sobocinska M.

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