PL
Analizowano wybrane markery chorobotwórczości 30 szczepów E. coli. Przy użyciu testów RPLA, w 10 szczepach wykazano syntezę enterotoksyny LT, a w 3 szczepach należących do typu serologicznego O157:H7 wykryto produkcję shiga toksyn E. coli – Stx1 i Stx2. Właściwości ß-hemolityczne in vitro zaobserwowano u 17 szczepów (57%). U 16 z nich (94%) stwierdzono obecność genów kodujących syntezę enterotoksyn lub jednocześnie enterotoksyn i shiga toksyny E. coli Stx2v. Stosując technikę PCR, w 21 szczepach (70%) określono genetyczne determinanty syntezy enterotoksyn LT, STa i/lub STb lub jednocześnie enterotoksyn i shiga toksyny E. coli Stx2v.
EN
This study included a description of enterotoxic and verocytotoxic activity of thirty strains of E. coli and their ability to produce ß-haemolysis in a ram blood medium. Enterotoxic and verocytotoxic activity was determined by using RPLA test. The synthesis of enterotoxin LT was observed in 10 strains and the production of E. coli shiga toxins type 1 - Sixl (1 strain) or type 2 (2 strains) was observed in 3 strains of serotype O157: H7. The ß-haemolytic characteristics in vitro were demonstrated by 17 strains (57%) isolated from pigs. Among them 16 (94%) were found to possess the genes that determine the enterotoxins or enterotoxins and E. coli shiga toxin Stx2v synthesis. Using the PCR technique, 21 strains (70%) were found to posses the genetic determinants of enterotoxins LT, STa and/or STb synthesis or enterotoxins and E. coli shiga toxin Stx2v synthesis. Further genetic study showed that the strains possessed the genes elt and estB were predominant (33%) among the toxic strains of E. coli isolated from piglets and calves.