In 50 domestic fowls: ducks, geese, turkeys and chickens, the content of vitamin C in the wall of glandular stomach, jejunum and caecum was determined. Vit. C was determined in tissues and blood plasma by the Roe-Kuether method, the level of an intermediate product of synthesis of D-glucuronic acid by the Bitter method, and the activity of L-gulono-y-oxidase active in the terminal stage of vitamin C synthesis by the Chatterjee method. The level of ascorbic acid synthesis was higher in the walls of the digestive tube in geese and ducks (321.1-294.2 mg/kg of tissue) than in turkeys and chickens (277.7-189.7 mg/kg of tissue). The authors conclude that there exists a close relationship between the synthesis of ascorbic acid, D-glucuronic acid and L-gulono-y-oxidase in domestic fowls. An increase of enzymatic activity of L-gulono-y-oxidase increases vitamin C biosynthesis. The synthesis of vitamin C in tissues of domestic fowls depends on the species and rearing conditions.