Polymorphism within the LDHA gene in the homing and non-homing pigeons

• Autorzy: Dybus A , Pijanka J , Cheng Y H , Sheen F , Grzesiak W , Muszynska M
• Języki publikacji: EN

Abstrakty

EN

A total of 445 domestic pigeons were genotyped for the lactate dehydrogenase (LDHA) gene. Crude DNA was isolated from blood samples and feathers. Two polymorphic sites were identified in intron 6: one near the splice donor site GT is called site H and the other near the splice acceptor site is called site B. Interestingly, the nucleotide changes of both these sites associate perfectly with the A and B alleles of HaeIII polymorphism: the A allele with nucleotide A of site H and nucleotide T of site B; while the B allele with nucleotide G of site H and nucleotide G of site B. In this study, we have identified the molecular difference between alleles A and B of the pigeon LDHA gene. The difference at site H in intron 6 explains the Hae III polymorphism. The frequencies of LDHA AB and LDHA BB genotypes between the analysed groups differ significantly (P < 0.001); the LDHA A allele was more frequent in the groups of pigeons with elevated homing performance (P < 0.001). The functional difference may be due to the change at site B, the potential splice branch site. Since LDHA activity is associated with the homing ability, it is possible that during the process of selection for the homing ability, the LDHA A allele has been selected, and is more prevalent in the top-racing groups.

Słowa kluczowe

EN

lactate dehydrogenase; pigeon; polymorphism; gene; feather; genotype; domestic pigeon; Columba livia var.domestica; nucleotide; polymerase chain reaction; allele; blood sample

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 1
• Opis fizyczny: p.63-66,fig.,ref.

Twórcy

autor

Dybus A

• Laboratory of Molecular Cytogenetics, Agricultural University of Szczecin, Doktora Judyma 12, 71-460 Szczecin, Poland

autor

Pijanka J

• Laboratory of Molecular Cytogenetics, Agricultural University of Szczecin, Doktora Judyma 12, 71-460 Szczecin, Poland

autor

Cheng Y H

• Department of Animal Science, National I-Lan University, Taiwan, Republic of China

autor

Sheen F

• Taiwan Genome Sciences, Nan Kang District, Taipei, Taiwan, Republic of China

autor

Grzesiak W

• Laboratory of Biostatics, Agricultural University of Szczecin, Szczecin, Poland

autor

Muszynska M

• Laboratory of Molecular Cytogenetics, Agricultural University of Szczecin, Doktora Judyma 12, 71-460 Szczecin, Poland

Bibliografia

• Apple FS, Rogers MA, 1986. Skeletal muscle lactate dehydrogenase isoenzyme alterations in men and women marathon runners. J Appl Physiol 61: 477—481.
• Bello N, Francino O, Sanchez A, 2001. Isolation of genomic DNA from feathers. J Vet Diagn Invest 13: 162-164.
• Cadefau J, Casademont J, Grau JM, Fernandez J, Balaguer A, Vernet M, et al. 1990. Biochemical and histochemical adaptation to sprint training in young athletes. Acta Physiol Scand 140: 341-351.
• Chaplin SB, Munson MM, Knuth ST, 1997. The effect of exercise and restraint on pectoral muscle metabolism in pigeons. J Comp Physiol (B) 167: 197-203.
• Dybus A, Kmieć M, 2002. PCR-RFLPs within the domestic pigeon (Columba livia var. domestica) lactate dehydrogenase (LDH-A) gene. J Appl Genet 43: 501-504.
• Holmes RS, 1972. Evolution of lactate dehydrogenase genes. FEBS Lett 28: 51-55.
• Li SSL, 1998. Structure, regulation and evolution of vertebrate lactate dehydrogenase genes. Zool Stud 37: 1-6.
• Mannen H, Tsoi SC, Krushkal JS, Li WH, Li SSL, 1997. The cDNA cloning and molecular evolution of reptile and pigeon lactate dehydrogenase isozymes. Mol Biol Evol 14: 1081-1087.
• Markert CL, Shaklee JB, Whitt GS, 1975. Evolution of a gene: Multiple genes for LDH isozymes provide a model of the evolution of gene structure, function and regulation. Science 189: 102-114.
• Tsuji S, Qureshi MA, Hou EW, Fitch WM, Li SS, 1994. Evolutionary relationships of lactate dehydrogenase (LDHs) from mammals, birds, an amphibian, fish, barley and bacteria: LDH cDNA sequences from Xenopus, pig, and rat. Proc Natl Acad Sci USA 91 : 9392-9396.
• Van Hall G, Gonzalez-Alonso J, Sacchetti M, Saltin B, 1999. Skeletal muscle substrate metabolism during exercise; methodological considerations. Proc Nutr Soc 58: 899-912.
• Van Hall G, 2000. Lactate as a fuel for mitochondrial respiration. Acta Physiol Scand 168: 643-656.