Zastosowanie testu RT-nested-PCR do wykrywania materialu genetycznego wirusa grypy swin w materiale klinicznym

• Autorzy: Kowalczyk A , Markowska-Daniel I. , Pejsak Z.

Warianty tytułu

EN

Application of RT-nested-PCR for RNA detection of swine influenza virus in clinical samples

• Języki publikacji: PL

Abstrakty

EN

Pigs serve as major reservoirs of H1N1 and H3N2 influenza viruses which are endemic in pig populations worldwide and are responsible for one of the most prevalent respiratory diseases in pigs. Therefore the early detection and identification of such events are paramount in monitoring the spread of influenza viruses. Transfer of influenza A viruses from animal hosts to man may lead to the emergence of new human pandemic strains. The aim of the study was to prove the matrix gene useful for reverse transcription nested-PCR as sensitive and specific for the detection of swine influenza A viruses in clinical samples. 75 RNA-virus positive samples out of 235 samples, including nasal swabs, lung tissues and whole blood samples, were detected by RT-n-PCR. Using conventional virology method we isolated 33 SIV strains in embryonated chicken SPF eggs. The results of PCR were 100% in agreement with those of virus isolation. The limit of detection of SIV was 10-7 EID50/0.1 ml. These results demonstrate the usefulness of RT-n-PCR for the detection and identification of influenza A virus in clinical material.

Słowa kluczowe

PL

trzoda chlewna; swoistosc; metoda RT-nested PCR; wykrywanie wirusow; metody; wirus influenzy swin; czulosc

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 07
• Opis fizyczny: s.810-814,rys.,fot.,tab.,bibliogr.

Twórcy

autor

Kowalczyk A

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Markowska-Daniel I.

autor

Pejsak Z.

Bibliografia

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