Zastosowanie analizy makrorestrykcyjnej i reakcji PCR w odroznianiu bezplazmidowych szczepow Bacillus anthracis od szczepow przejsciowych [Bacillus sp. Ba 813]

• Autorzy: Niemcewicz M , Bartoszcze M.

Warianty tytułu

EN

Applying macro restriction analysis and PCR in differentiating plasmid cured strains of Bacillus anthracis from transitional strains [Bacillus sp. Ba 813]

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to use selected genetic analysis to differentiate plasmid cured strains of Bacillus anthracis from transitional strains (Bacillus sp. Ba 813). Two different research techniques (macro restriction analysis and PCR) were used and they confirmed that the PCR technique facilitated detecting the presence of the Ba 813 chromosomal sequence in both strains (plasmid cured Bacillus anthracis strain and Bacillus sp. Ba 813). This result indicated that applying the PCR method to differentiate plasmid cured Bacillus anthracis strains from Bacillus sp. Ba 813 was not possible. The macro restriction method, however, facilitated a precise differentiation of plasmid cured Bacillus anthracis strains from transitional Bacillus sp. Ba 813.

Słowa kluczowe

PL

Bacillus anthracis; metoda makrorestrykcji; laseczki waglika; lancuchowa reakcja polimerazy; szczepy przejsciowe; szczepy bezplazmidowe; bakterie; roznicowanie; metody

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 06
• Opis fizyczny: s.658-662,rys.,bibliogr.

Twórcy

autor

Niemcewicz M

• Wojskowy Instytut Higieny i Epidemiologii, ul.Lubelska 2, 24-100 Pulawy

autor

Bartoszcze M.

Bibliografia

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