Experimental hypothyroidism increases content of collagen and glycosaminoglycans in the heart

• Autorzy: Drobnik J. , Ciosek J. , Slotwinska D. , Stempniak B. , Zukowska D. , Marczynski A. , Tosik D. , Bartel H. , Dabrowski R. , Szczepanowska A.
• Języki publikacji: EN

Abstrakty

EN

The connective tissue matrix of the heart remains under regulatory influence of the thyroid hormones. Some conflicting data describe the connective tissue changes in subjects with thyroid gland disorders. The aim of the study was to assess the changes of the connective tissue accumulation in the heart of rats in the state of hypothyroidism and to answer the question whether TSH is involved in mechanism of the observed phenomena. Hypothyroidism in rats was induced by methylotiouracil treatment or by thyreoidectomy. The thyroid hormones [freeT3 (fT3), freeT4 (fT4)] and pituitary TSH were measured in plasma with radioimmunological method. The glycosaminoglycans (GAG) and total collagen were measured in heart muscle of both left and right ventricles. Cells from the rat’s heart were isolated and cultured. The cells were identified as myofibroblasts by electron microscopy method. The effects of TSH in concentrations ranging from 0.002 to 20 mIU/ml, on connective tissue accumulation in heart myofibroblasts cultures were tested. The primary hypothyroidism was developed both in groups with thyroidectomy and with methylthiouracil. The levels of fT3 and fT4 both in rats with thyreoidectomy and animals treated with methylthiouracil were decreased and TSH level in these two experimental groups was elevated. In the heart of the rats with experimental hypothyroidism increased content of both GAG and collagen was found. Myofibroblast number in culture was increased by TSH. Regardless of the method of its induction, hypothyroidism increased collagen and GAG contents in the heart. TSH is not involved in regulation of collagen and glycosaminoglycans accumulation in the heart of rats affected with primary hypothyroidism.

Słowa kluczowe

EN

hypothyroidism; collagen; glycosaminoglycan; thyroid hormone; thyroid gland; thyroxine; thyroidectomy; triiodothyronine; heart; connective tissue; methylthiouracil; myofibroblast culture

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 3
• Opis fizyczny: p.57-62,fig.,ref.

Twórcy

autor

Drobnik J.

• Medical University of Lodz, 60 Narutowicza Street, 90-136 Lodz, Poland

autor

Ciosek J.

autor

Slotwinska D.

autor

Stempniak B.

autor

Zukowska D.

autor

Marczynski A.

autor

Tosik D.

autor

Bartel H.

autor

Dabrowski R.

autor

Szczepanowska A.