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2006 | 62 | 05 |

Tytuł artykułu

Syntetyczny plytkowy czynnik aktywujacy [PAF] jako skladnik rozcienczalnika dla nasienia knura

Autorzy

Warianty tytułu

EN
Synthetic platelet activating factor [PAF] as a boar semen extender component

Języki publikacji

PL

Abstrakty

EN
The aim of this study was to investigate the effects of PAF addition on selected motility characteristics and plasmalemma integrity of boar spermatozoa following liquid storage in a boar semen extender, Kortowo-3 (K-3), supplemented with lipoprotein fractions extracted from hen egg yolk (LPFh) or lyophilized lipoprotein fractions extracted from ostrich egg yolk (LPFo), at 5°C and 16°C. Sperm motility was evaluated using a computer system (CASA). The determination of AspAT activity in sperm extracts as well as fluorescent analysis, with a fluorochrome, Hoechst 33258, were used to assess the plasmalemma integrity overlying the middle-piece and acrosomal regions of spermatozoa, respectively. It was confirmed that the addition of exogenous PAF to K-3 extender containing LPFh or LPFo had a beneficial effect on the sperm quality parameters during storage at 5°C or 16°C. This phenomenon was manifested by an increase in motility and survivability of spermatozoa. The use of LPFh or LPFo as a component of boar semen extender had a protective effect on the plasmalemma integrity overlying the middle-piece and acrosomal regions of PAF-treated spermatozoa.

Wydawca

-

Rocznik

Tom

62

Numer

05

Opis fizyczny

s.560-562,rys.,tab.,bibliogr.

Twórcy

autor
  • Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 5, 10-718 Olsztyn
autor

Bibliografia

  • 1. Bielański W.: Rozród zwierząt. PWN, Warszawa 1974.
  • 2. Ciereszko A., Glogowski J., Demianowicz W., Strzeżek J.: Stimulation of aspartate aminotransferase from animal semen by pyridoxal 5'-phosphate. Anim. Reprod. Sci. 1994, 34, 327-341.
  • 3. Fraser L., Lecewicz M., Strzeżek J.: Effect of extender composition and storage temperatures on motility of preserved boar semen. J. Anim. Feed Sci. 2002, 11, 661-669.
  • 4. Hough S. H., Parks J. E.: Platelet-activating factor acetylhydrolase activity in seminal plasma from the bull, stallion, rabbit, and rooster. Biol. Reprod. 1994, 50, 912-916.
  • 5. Kordan W.: Właściwości acetylohdrolazy płytkowego czynnika aktywującego (PAF-AH) z plazmy nasienia knura. Praca hab. Rozprawy i monografie nr 44, 2001, 1-53, Wyd. Uniwersytetu Warmińsko-Mazurskiego w Olsztynie.
  • 6. Kordan W., Strzeżek J.: Effect of platelet-activating factor (PAF) on motility parameters and plasmalemma integrity of boar spermatozoa. Anim. Sci. Pap. Rep. 2002, 20, 37-45.
  • 7. Kordan W., Strzeżek J., Fraser L.: Functions of platelet activating factor (PAF) in mammalian reproductive processes: a review. Pol. J. Vet. Sci. 2003, 6, 55-60.
  • 8. Kordan W., Wysocki P., Strzeżek J.: A polypeptide from protein complex of platelet activating factor acetylhydrolase (PAF-AH) of boar seminal plasma belongs to a family of adhesion proteins. Anim. Sci. Pap. Rep. 2004, 22, 341-346.
  • 9. Odeh A. I., Dascanio J. J., Caceci T., Bowen J., Eng L. A.: Effect of platelet activating factor (PAF) on stallion sperm motility, capacitation and acrosome reaction. Reproduction 2003, 126, 605-613.
  • 10. Parks J. E., Hough S. R.: Effect of platelet activating factor on the motility and acrosome reaction of bovie spermatozoa. Theriogenology 1990, 34, 903-912.
  • 11. Reinhardt J. C., Xiaoying Cui, Roudebush W. E.: Immunofluorescent evidence of the platelet-activating factor receptor on human spermatozoa. Fertil. Steril. 1999, 71, 941-942.
  • 12. Roudebush W. E., Diehl J. R.: Platelet-activating factor content in boar spermatozoa correlates with fertility. Theriogenology 2001, 55, 1633-1638.
  • 13. Soubeyrand S., Lazure C., Manjunath P.: Phospholipase A2 from bovine seminal plasma is a platelet-activating factor acetylhydrolase. Biochem. J. 1998, 329, 41-47.
  • 14. Snell W. J., White J. M.: The molecules of mammalinan fertilization. Cell 1996, 85, 629-637.
  • 15. Strzeżek J., Lecewicz M., Dziekońska A., Fraser L.: A simple metod of extraction of lipoprotein fraction from avian egg yolk-protective effect on cooled boar semen. Theriogenology 2005, 63, 496-497.
  • 16. Wolders H.: A novel technique for differential live-dead staining using fluorescence microscopy and fluorimetry. Proc. 11th Congress Anim. Reprod. Art. Insem. Dublin 1988, 4, 579.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-093725e6-17cb-4fd1-b3aa-9f0b2bdc29a8
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