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Czasopismo

1995 | 37 | 4 |

Tytuł artykułu

Regulation of the nitrite transport system in Chlorella fusca Shih. et Krauss

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Uptake of nitrite by C. fusca cells was considerable only in the light. Pre-starvation in the dark or permanent darkness was accompanied by very low rates of nitrite uptake. 2,4 dinitrophenol, an energy metabolism inhibitor, also inhibited nitrite uptake. Nevertheless, it is difficult to assume that nitrite uptake is an active process. Metabolic inhibitors having various targets in plant cell metabolism such as arsenate, cyanide, cyclohexamide and 6.methylpurine also inhibited nitrite uptake. A feedback inhibition of nitrite uptake was observed in the presence of glutamate or glutamine. The addition of NO3- or NH4+ competitively inhibited NO2- uptake. Therefore, NO2- uptake in C. fusca is conditioned by several factors.
PL
Zauważalne pobieranie azotynów przez komórki C. fusca ma miejsce tylko w świetle. Po wstępnej inkubacji w ciemności lub w warunkach nieprzerwanej ciemności stwierdzono niskie tempo pobierania azotynów. Pobieranie azotynów jest również hamowane przez 2,4 dinitrofenol. Pomimo to trudno jest uznać pobór azotynów za proces aktywny. Inhibitory metaboliczne działające na różne procesy metaboliczne komórek roślinnych, jak arseniany, cyjanki, cykloheksamid, 6.metylopuryna również hamują pobieranie azotynów. Sprzężenie zwrotne hamujące pobieranie azotynów stwierdzono w obecności glutaminianu lub glutaminy. Dodatek NO3- lub NH4+ hamuje konkurencyjnie pobór NO2- .Wskazuje to, że pobieranie NO2- przez C. fusca jest uzależnione od kilku czynników.

Wydawca

-

Czasopismo

Rocznik

Tom

37

Numer

4

Opis fizyczny

p.183-189,fig.

Twórcy

  • University of Assiut, Assiut, Egypt
autor

Bibliografia

  • Ali A.H., Al-Baiti A.R., Sachit E.O. and Rafiq S.N. 1988. The uptake of nitrite by Cladosporium herbarium and Chaetomium globosum. Zanco, 6, 63-72.
  • Ali A.H. and Hipkin C.R. 1985. Nitrate assimilation in the basidiomycete yeast Sporobolomyces roseus. J. General Microbiology, 131, 1867-1874.
  • Ali A.H. and Hipkin C.R. 1986. Nitrate assimilation in Candida nitratophila and other yeast. Arch. Microbiol., 144, 263-267.
  • Ali A.H. and Hipkin C.R. 1987. Some characteristics of inorganic nitrogen assimilation in the basidiomycete yeast Sporobolomyces roseus. Zanco, 5, 21-31.
  • Ali A.H. and Rafiq S.M. 1988. Uptake and generation of nitrite by Candida nitratophila. J. Biol. Sci. Res., 19, 13-28.
  • Ali A.H. and Rafiq S.M. 1989. The role of nitrogen sources in the regulation of nitrate reductase (NR) and nitrite assimilation in the yeast Rhodotorula glutinis. Zanco, 1, 3-19.
  • Chang H.C.P., Mulkins G.Y., Dyet J.C. and Sorger G.J. 1975. Enzymatic and nonenzymatic reduction of nitrite by extract of Neurospora crassa. J. Bacteriol., 123, 755-758.
  • Cresswell R.C. and Syrett P.J. 1982. The uptake of nitrite by the diatom Phaeodactylum. Interaction between nitrite and nitrate. J. Exp. Bot., 33, 1111-1121.
  • De La Haba P., Aguera E. and Maldonado J.M. 1988. Development of nitrogen-assimilating enzymes in sunflower cotyledons during germination as affected by the exogenous nitrogen source. Planta, 173, 52-57.
  • Eppley R.W. and Coatsworth J.L. 1968. Uptake of nitrate and nitrite by Ditylum brightwelli - kinetics and mechanisms. J. Phycol., 4, 151-156.
  • Everest S.A., Hipkin C.R. and Syrett P.J. 1984. The effect of phosphate and flavin adenine dinucleotide on nitrate reductase activity of some unicellular marine algae. J. Exp. Biol. Ecol., 76, 263-273.
  • Fuggi A. 1989. Competition between nitrate and nitrite as a tool to study regulation of nitrate metabolism by ammonium in the microalga Cynidium caldarium. Plant Physiol. Biochem., 27, 563-568.
  • Fuggi A. 1990. Uptake of inorganic nitrogen compounds in an acidophilic alga. In: Ulrich W.R., Rigano C., Fuggi A. and Aparicio P.J. (eds) Inorganic nitrogen in plants and microorganisms - uptake and metabolism. Berlin-Heidelberg-New York, Springer, 66-72.
  • Madueno F., Flores E. and Guerrero G.M. 1987. Competition between nitrate and nitrite uptake in the cyanobacterium Anacystis nidulans. Biochim. Biophys. Acta, 896, 109-112.
  • Maldanado J.M., Aguerea E. and De La Haba P. 1990. Nitrate and nitrite utilization by sunflower plants. In: Ulrich W.R., Rigano C., Fuggi A. and Aparicio P.J. (eds) Inorganic nitrogen in plants and microorganisms - uptake and metabolism. Berlin-Heidelberg-New York, Springer, 159-164.
  • Metzner H., Rau H. and Senger H. 1965. Untersuchungen von Synchronisierbarkeit einzelner Pigmentmangel-Mutanten von Chlorella. Planta, 65, 186-194.
  • Pateman J.A. and Kinghorn J.R. 1976. Nitrogen metabolism. In: Smith J.E. and Berry D.R. (eds) The filamentous fungi, vol. 2. London, Edward Arnold, 159-237.
  • Rajaskhar V.K. and Mohr H. 1986. Effect of ammonium and nitrate on growth and appearance of nitrate and nitrite reductase in dark- and light-grown mustard seedlings. Planta, 169, 594-599.
  • Schloemer R.H. and Garrett R.H. 1974. Uptake of nitrite by Neurospora crassa. J. Bacteriol., 118, 270-274.
  • Stein J.R. 1966. Growth and mating of Gonium pectorale (Volvocales) in defined media. J. Phycol., 2, 23-28.
  • Thacker A. and Syrett P.J. 1972. The assimilation of nitrate and ammonium by Chlamydomonas reinhardii. New Phytol., 71, 423-433.
  • Ulrich W.R. 1987. Nitrate and ammonium uptake in green algae and higher plants: Mechanism and relationship with nitrate metabolism. In: Ulrich W.R., Aparicio P.J., Syrett P.J. and Castillo F. (eds) Inorganic nitrogen metabolism. Berlin-Heidelberg-New York, Springer, 32-38.
  • Vega J.M. and Menacho A. 1990. Regulation of inorganic nitrogen metabolism in Chlamydomonas reinhardtii. In: Ulrich W.R., Rigano C., Fuggi A. and Aparicio P.J. (eds) Inorganic nitrogen in plants and microorganisms - uptake and metabolism. Berlin-Heidelberg-New York, Springer, 159-164.

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Bibliografia

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